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How much of a 5x buffer and how much water should be added to make 1 L of a 1x solution?
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- How much of a 10x buffer should be added to 900ml of water to make a 1x solution?If 4 mL of 1 M NaOH is added to 100 mL buffer, would it still be a usable buffer according to the conventions? Explain why or why not.50X TBE is 87 mM Tris-borate and 4 mM EDTA at pH 8.3. What is the molarity of each of the chemicals in 1X TBE buffer?
- What is the relationship betweenpKa and the useful range of a buffer?Calculate the volume of TAE buffer that you will need to prepare a 100ml solution of 1X strength if you only have a 50X concentrated solution available.Calculate pH of buffer prepared by mixing; 10ml of 0.10M of CH3COOH and 20ml of 0.10M of CH3COONa.
- You need to prepare an acetate buffer of pH 5.43 from a 0.621 M acetic acid solution and a 2.95 M KOH solution. If you have 730 mL of the acetic acid solution, how many milliliters of the KOH solution do you need to add to make a buffer of pH 5.43? The pKa of acetic acid is 4.76. Be sure to use appropriate significant figures.A 500 mL sample of a 0.100 M formate buffer, pH 3.75, is treated with 5 mL of 1.00 M KOH. What is the pH following this addition?What would be the working stock concentration (in molarity) if you diluted 250 uL of a stock concentration of 10 uM in a total volume of 1 mL?