The codon chart below shows that adenine-uracil-guanine (AUG) codes for the amino acid methionine, and cytosine- adenine-guanine (CAG) codes for glutamine in humans. RNA Codon Chart Alanine Tyrosine Stop Valine GU Cystoine Stop GTyptophan G Arginine Leucine Serine 8663 Lysine Proline Asparagine Select the two amino acids that those two codons code for in carrots. O glutamine O isoleucine O methionine O serine O valine uaud Leucine Giycine Glutami acid Histidine Aspartic acid Glutamine Arginine aonejos Methionine

Determine which amino acid is formed from the following mRNA codon: GUA.    a aspartic acid b glutamic acid c phenylalanine d valine

E GFL D A Alanine STOP A V A GU AC Valine U G G Tryptophan STOP Arginine Leucine Serine A A UG /ACUGACU K Lysine Proline Asparagine M RQH START T Glycine Leucine Serine Phenyl- Glutamic acid Histidine Glutamine Arginine Threonine Methionine

Suppose that a protein has the amino acid sequence Met‑Tyr‑Asn‑Val‑Arg‑Val‑Tyr‑Lys‑Ala‑Lys‑Trp‑Leu‑Ile‑His‑Thr‑Pro A researcher plans to make a set of probes to screen a cDNA library for the sequence that encodes this protein. Her probes should be exactly 18 nucleotides in length. Consult the codon table. Select which amino acids in the protein should be used to minimize degeneracy when designing the probes. Val‑Tyr‑Lys‑Ala‑Lys‑Trp Met‑Tyr‑Asn‑Val‑Arg‑Val Arg‑Val‑Tyr‑Lys‑Ala‑Ly Trp‑Leu‑Ile‑His‑Thr‑Pro Calculate the number of different probes that must be synthesized in order to determine the exact cDNA sequence that specifies the region of the protein selected. number of probes:

If a codon GCA codes for the amino acid alanine in a prokaryote, what will it code for in a eukaryote? _____

An RNA polymer is made by using the enzyme polynucleotide phosphorylase with equal quantities of CTP and GTP. When this RNA is used in an in vitro translation system, all of the following amino acids could be incorporated into a newly made polypeptide, except: Codon Table Second position C UUU UCU UAU UGU phe tyr сys UUC UCC UAC UGC ser UAA Stop UGA Stop UAG Stop UGG trp UUA UCA UUG UCG CUU CCU CAU CGU leu his ССС pro ССА CỤC САС CGC arg CỦA САА CGA gln CUG CCG CAG CGG AUU ACU AAU AGU asn ser AUC ile ACC thr АCА AAC AGC AUA AAA lys AAG AGA arg AUG met ACG AGG GUU GCU GAU GGU asp GUC GCC ala GCA GẠC GGC val gly GUA GAA GGA glu GUG GCG GAG GGG glycine (Gly) histidine (His) proline (pro) alanine (Ala) arginine (Arg) Third position (3'-end) AGUCAG First position (5'-end)

Consider the peptide Asp-Lys-Phe-Glu-Asn-Tyr-Gln-Val-Cys. In a single beaker, you treat this peptide with 2 proteases. One protease cleaves at the N-terminus of aromatic R groups and the other cleaves at the C-terminus of polar, non-ionizable R groups. Following the enzymatic digestion, you want to separate your peptide fragments so that you can identify them. You choose to separate the fragments using an anion exchange column. Beginning at pH=6 you apply your peptide fragments to the column and you gradually decrease the pH of the column stopping the separation when the pH of the column equals 4. Omitting chemical structures, write the amino acid sequence of the peptide fragments that are produced from this digest. Write the order that these fragments will elute from the column (if at all). (Relevant pKa values are: 2.1, 3.8, 4.3, 8.3, 9.6, 10.1, and 10.5)

UCAGUCAGUOPODOTO E GFL A Alanine GU STOP Tyrosine A Stop C STOP V A Valine GU Cysteine G Stop G Tryplophan Arginine G A C A U Leucine Serine A UG Lysine Proline Asparagine GACUGACU H. MIR START Glycine Serine Leucine Glutamic Phenyl acid Aspartie acid Histidine RSK Glutamine Arginine Methionine Isoleucine Threonine

Group I and II introns are present in the following classes of RNAS. MRNAS O MIRNAS SİRNAS TRNAS

Which amino acid sequence will be generated?  5'-GAAUGUCUUCGUUAUUGAUGUAGAA-3'