The cell concentration measured in samples taken at certain intervals from a microorganism growth medium is given below. Calculate the doubling time and specific growth rate of this microorganism.

Q: Discuss why microorganism presents in the nature environment has a longer lag phase and shorter…

A: The microorganisms present in the environment are continually replicating and utilizing the…

Q: What is sterilization? Give specific examples of media which are sterilized by the following…

A: All microbes, such as bacteria, virus, and fungi, requires some essential nutrients for growth. The…

Q: Describe how you would prepare a dilution series of a 1 x 107 CFU/mL culture to the 10-8 dilution…

A: Serial dilutions is a process of making step by step dilutions of a substance. It is formed by…

Q: During cell couting, when the data are plotted in an ordinary graph or on a cross-section paper, why…

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Q: The optimum growth pH for a microorganism is usually:  4,5,7 or 8

A: pH is the scale on which the acidity or alkalinity  is measured. It is the scale between 1 to 14.…

Q: What conclusion would you draw if the numbers of bacterial colonies in were the same on the control…

A: Bacteria are unicellular and prokaryotic organisms that live in wide variety of environments. It…

Q: What concentration of agar is used in a semisolid medium for motility determination?

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Q: discuss the significance of the different aseptic techniques utilized in maintaining sterility of…

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Q: Calculate the growth rate and generation time of a culture that increases in the exponential phase…

A: Growth refers to changes in viable cell count as compared to time. The time it takes for bacteria to…

Q: Differentiate the growth curve exhibited by the same inoculum under the following conditions.  Draw…

A:

Q: Calculate the growth rate and generation time of a culture that increases in the exponential phase…

A: The growth of bacteria over a period of time is represented by a growth curve. This curve tells the…

Q: n this step, wash the cells with ice-cold PBS. Drain the PBS, then add ice-cold lysis buffer.…

A: The question you have asked lacks some background information about what you are trying to do, But…

Q: What substance is produce by a microorganism that is capable of the inhibiting the growth of other…

A: Introduction Microorganisms are an organism that can be seen only through a microscope.…

Q: Show the calculations required to make up 250mlof a stock solution of this chemical that will then…

A: Introduction :- Dilution is the process through which we can make a solution less concentrated by…

Q: Explain the significance of aseptic culture technique in growing microorganism

A:

Q: Provide comparison and illustration of expected growth curves from a pure culture with the…

A: 1. The growth curve from a pure culture represents the number of live cells in a bacterial…

Q: What are the difference in the movement of microorganisms under wet mount from hanging drop…

A: There are some plus point for both the techniques like the wet Mount and hanging drop method…

Q: Are you able to identify the lag, log, stationary and death phase in using this growth curve?

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Q: A disk-diffusion test using E. coli gave the following results:  Disinfectant         Zone of…

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Q: Explain why the following steps are necessary during subculturing:  Cooling the inoculating loop…

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Q: Describe the five phases of a microbial growth curve observed when microbes are grown in a batch…

A: Introduction: Bacteria is unicellular prokaryotic organism that can survive in any kind of…

Q: Shown below is the growth of E. coli in nutrient agar plates after exposure to different doses of UV…

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Q: Describe how to most easily calculate the generation time for a population of bacteria in a batch…

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Q: An organism capable of growing only in a low salt concentration, such as saline, is considered…

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Q: All of the following are valid measurements of growth except: A. Counting viable cells B.…

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Q: Calculate the doubling time of a culture of E. coli that is started with 3x105 CFU/mL and grows to…

A: Given: No = 3x105 CFU/mL (number of bacteria at the beginning of a time interval) Nt = 4.6x108…

Q: use these OD numbers to plot growth curve for E. coli K12– and estimate generation time for this…

A: The growth curve for E.coli K12 is plotted as follows:

Q: Which has a greater impact on the survival of an organism, temperatures above its maximum…

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Q: In disc diffusion studies, where will the lowest concentration of the tested chemical be in the agar…

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Q: Give two reasons for why heat fixing is used?  Adherence of bacterial cells to the slide…

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Q: based on the attached photo, compute the cfu/ml of 42 colonies at plate 10^5. show the complete…

A: To calculate the colony-forming unit per ml (cfu/ml) following formula can be used. CFU/ml= no. of…

Q: On agar plate does each discrete colony represent the growth of one cell? Explain your answer. Why…

A: Question - On agar plate does each discrete colony represent the growth of one cell? Explain your…

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Q: SEPARATION OF COMPONENTS BY CENTRIFUGATION Consider the following subcellular components and their…

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Q: Using a solid line draw a standard bacterial growth curve. Represent all the phases by numbering…

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Q: What are two advantages of filtration in microbial control? Explain how the filtration process…

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Q: Are you able to identify the lag, log, stationary and death phase in using this growth curve? Are…

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Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.