Give two reasons for why heat fixing is used? Adherence of bacterial cells to the slide Denaturing of bacterial enzymes to preserve cellular integrity
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5. Give two reasons for why heat fixing is used?
Adherence of bacterial cells to the slide
Denaturing of bacterial enzymes to preserve cellular integrity
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- 1. Determine the minimum inhibitory concentration and the minimum exposure time given the following data: Number of colonies of E. coli on Nutrient Agar plates Exposure time (in minutes) Concentration of ethanol 10 15 Distilled water +++ +++ +++ 40% +++ ++ + 70% 95% Minimum inhibitory concentration: Minimum exposure time:8. Pigment or color (e.g.: opaque, translucid, red, yellow, rose, violet, etc..) is also another characteristic that can be used to identify your microorganism in a slant Thus, write down the name of three microorganisms (using scientific notation: Escherichia coli) that present a pigmented pattern of growth in a slant (write down the color of each pigment) and the name of the disease that they may cause. F10 F12 F6 %24 6. R. Home Enter K F G A Shift C Page Up Page Down Alt Ctrl Alt Σ8. Pigment or color (e.g.: opaque, translucid, red, yellow, rose, violet, etc..) is also another characteristic that can be used to identify your microorganism in a slant. Thus, write down the name of three microorganisms (using scientific notation: Escherichia coli) that present a pigmented pattern of growth in a slant (write down the color of each pigment) and the name of the disease that they may cause. 200%- 927 PM 0 日 2/7/2021 21 DELL Insert Delete F11 F12 PrtScr F9 F10 F7 F8 F6 F3 F4 F5 Esc F1 F2 Backspace & # 2$ 8 4 1 2 P Home R T E Enter K Shift V B Page Down Page Up Alt Ctrl Alt THome ノ/ *00 Σ
- 29. Identify a FALSE statement from the following, Group of answer choices bacterial growth is related to nutrient concentration and availability sulfur is essential in growth medium for some amino acid synthesis none of the above is an false statement nutrients uptake can be via active or passive process culture medium must be sterile before inoculation 30. Identify a CORRECT statement regarding penicillin allergy? Group of answer choices the hypersensitive reactions are mediated by IgE penicillin is degraded to penicilloyl and other breakdown products all of the statements are correct penicilloyl haptens trigger most allergic reactions anaphylactic shock is the immediate danger penicillin can provoke1.Define Bioprospecting.2. What properties in microorganisms were researchers at SINTEF and NTNU looking for?3. What made them select Micrococcus luteus as a candidate organism? 4. Describe the steps involved in genetic engineering, prior to commercial production of the potential `UVAblue’ sunscreen.5. What damage do long wave UV radiations induce in human cells?Explain 2 factors that influence microbial growth.
- 8. A. Use Excel (or another graphing program) to draw the growth curve, In (X/X.) vs time, for bacteria grown in a 20 L suspension cell culture, given the following data: - initial concentration: 0.120 gdw cells/L Also report: - lag time: 1.5 hours - mass doubling time during exponential growth: 250 minutes - duration of exponential growth phase: 1 day (24 hours) - negligible time in the deceleration phase - 13 hours in the endogenous metabolism phase with no change in cell concentration - cell death rate with k = 0.0178 min -¹. B. What is the specific growth rate, µ? C. What is the maximum concentration of cells in the reactor? (gdw cells/L) and when does this occur? D. Other than time zero or the end of lag phase, at what time is the concentration of living cells in the reactor equal to the initial concentration of 0.120 gdw/L?1. Which of the following micro-organisms are the most resistant to heat sterilization? a. Vegetative bacteria (e.g., Samonella spp.) b. Spore-forming mesophilic bacteria (e.g., Clostridium botulinum) c. Spore-forming thermophilic bacteria (e.g., Bacillus stearothermophilus) d. Toxicogenic bacteria (e.g., E. coli O157:H7) e. All of the above are equally resistant to heat sterilization.nt ● ● S ● Paragraph F 2. To determine if the type of agar affects bacterial growth, a scientist cultures on fou different types of agar. Five petri dishes are set up to collect results: One with nutrient agar and E. coli One with mannitol-salt agar and E. coli One with MacConkey agar and E. coli One with LB agar and E. coli Styles One with nutrient agar but NO E. coli Ed
- 1. Escherichia coli but not Pyrolobus fumarii will grow at 40°C, while P. fumarii but not E. coli will grow at 110°C. What is happening (or not happening) to prevent growth of each organism at the non-permissive temperature?. A certain medium has the following composition: Glucose 15 g Yeast extract 5 g Peptone 5 g KH2PO4 2 g Distilled water 1,000 ml Tell what chemical category this medium belongs to, and explain why this is true. How could you convert Staphylococcus medium (table 3.6A) into a nonsynthetic medium? a. Name four categories that blood agar fits. Alpha hemolysis Beta hemolysis Gamma Alpha prime Name four differential reactions that TSIA shows. Observe figure 3.15. Suggest what causes the difference in growth pattern between nonmotile and motile bacteria. Explain what a medium that is both selective and differential does, using figure 3.18. a. What kind of medium might you make to selectively grow a bacterium that lives in the ocean? MSA Mannitol salt agar One that lives in the human stomach? Why are intestinal bacteria able to grow…Why might clinical medicine have an interest in understanding bacterial cell division at the molecular level? Explain why a hyperthermophile would probably not be a human pathogen. Describe four factors that may have an influence on the effectiveness of an antimicrobial treatment. Explain why 70% or 80% alcohol is more effective than 100% alcohol in controlling microorganisms.
