discuss the significance of the different aseptic techniques utilized in maintaining sterility of the cell culture lab.
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discuss the significance of the different aseptic techniques utilized in maintaining sterility of the cell culture lab.
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- Provide three reasons why the use of aseptic technique is essential when handling microbial cultures in the laboratory.Identify Four (4) possibilities in cell culture practices that able affect the quality and yieldof biomedical production in the industry. use computer typing to answer thisExplain the significance of aseptic culture technique in growing microorganisms.
- Describe the difference between batch and continuous culture techniquesWhat is the principle of freezing of cell lines and primary cells.Describe the general protocol for freezing cultured cells.In detail.Explain why is it important to follow proper precautions and use aseptic technique when working with clinical samples.
- (1) why can't we say "sterile" technique (2) how are aseptic technique similar and different in the lab and Healthcare field?Be specific and explain at least 2 differences and two similarities. (3) You are asked to develop a method of transfer an unknown organism from a liquid broth to a solid petri dish.list each step that you would have to take .be specificYou are transferring a bacterial culture from a tube of broth to a Petri dish containing trypticase soy agar. Please list the steps that are necessary to make the transfer successfully and with minimal risk. of contamination.Can you please discuss the principles of using Motility Test Medium for the detection of bacterial motility. Focus on the components of the medium and the purpose of each component. Note: You may refer to different culture media manuals like Difco, BBL, etc.
- Refer to the provided image drawn by a student trying to plan out their serial dilution protocol. The student diluted the original culture into bottle A and then diluted it further into B as shown. The student then proceeded with plating out 0.1ml of the culture from bottle B onto the plate (Note: plate A shows the 0.1ml that was plated, no further dilutions were done). If 13 colonies grew on plate A, help the student figure out how many CFU (colony forming units) were in the original culture? Select one: a.1,300 b.13,000 c.None of the Above d.130,000 e.1,000,0001a.) How pH of the culture medium affects the cell culture? Please give examples of low pH and high-pH culture conditions. 1b.) Describe the difference between defined and undefined cell culture media. What are the advantages and disadvantages of both of them?The thermal death time in an autoclave at 121oC for a microbial culture was thirteen minutes. The thermal death time for another sample of the same culture in a hot-air oven at 121oC was twenty-one minutes. Use your knowledge of what thermal death time measures to identify which method of heating was more effective against this microbial culture. The hot-air oven treatment was more effective. The autoclave treatment was more effective. Both thermal death times were equally effective. Not enough information is provided to determine effectiveness.
