Answered: Table 1. PCR Components Final… | bartleby

Aquaculture Science

Aquaculture Science

3rd Edition

ISBN:9781133558347

Author:Parker

Publisher:Parker

Chapter2: Aquatic Plants And Animals

Section: Chapter Questions

Problem 2KA

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Answer the table below:

28
Table 1. PCR Components
Volume
Final
Concentration
Volume
for 1x
Initial
PCR Components
for 13x
Concentration [C1]
[C2]
[V1]
[V2]
sterile distilled H20
PCR Buffer
Magnesium
Chloride (MgCl2)
DNTP Mix
10x
1x
50 mM
1.5 mM
0.4 mM
0.3 µM
0.3 μΜ
10/25 µL
100 ng
10 mM
Forward Primers
Reverse Primers
Taq Polymerase
genomic DNA
template
10 µM
10 μΜ.
5U/µL
100 ng/µL
Total
25.0µL

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