Describe your color observations of the Nitrate test. a. after adding Reagents A, B (and/or znic): b. Did the organism reduce nitrate? (yes or no) c. Is the final product nitrate, nitrite or ammonia/nitrogen gas? Question 13 4 pts Based on your observations of the SIM test: a. Did sulfur reduction occur (yes/no)? b. Did the organism produce the enzyme tryptophanase (yes/no)? c. Is the organism positive or negative for the motility test?

Q: The following set of data are measurements of zones of inhibition (in mm) from a Kirby-Bauer test.…

A: The Kirby-Bauer test, also known as the disc diffusion test, is a standard method used to determine…

Q: Match the chemical reaction to the visual effect of the product/biochemical test description…

A: 1. Assay's colour change relies on intial reduction reaction resulting in a black precipitate if the…

Q: Cemical analysis of a patient’s urine using a reagent strip gave negative protein and negative…

A: Urine reagent strips are used for qualitative analysis of abnormal conditions such as the presence…

Q: 4. acetic acid on the ammonium sulfate precipitation of albumin 5. presence of precipitate during…

A: Molecular techniques are important tools that help in filtration, isolation, and identification of…

Q: What is the dilution factor in the task given if the total viable cells is 140 [28×5(squares)] and…

A: The dilution factor is a measure of how much a solution has been diluted or concentrated. It is…

Q: Ex. 5-4 Catalase 1) In the Catalase test (5-4), if bacteria was added to hydrogen peroxide on the…

A: The study of tiny living creatures such as bacteria, viruses, fungus, algae, and protozoa is known…

Q: Why does the oxidase test have to be read quickly?

A: The Oxidase test is a biochemical test for the identification of bacteria. The test is mainly…

Q: Match the test with the appropriate reagent/ chemical  a. DNA test b. Paper chromatography  c.…

A: Genetic material is nothing but the sequence of nucleic acids which is called as DNA. It contains…

Q: C The results of Motility Indole Ornithine (MIO) test - after the addition of Kovac's reagent - are…

A: Tryptophan is an essential amino acid. Conversion of tryptophan into metabolic products is mediated…

Q: What is the result of tube A in this Voges-proskauer test? What are the reagents of this test? What…

A: Voges-proskauer test is performed to differentiate between facultative anaerobic enteric bacteria.

Q: Lucas's unwashed, dirty hand produces 947 CFU when swabbed onto a nutrient agar plate, while his…

A: CFU stands for colony forming unit.

Q: Does Escherichia coli provide positive or negative results for the lysine decarboxylase and…

A: Ornithine decarboxylase test detects whether bacteria uses ornithine amino acid for carbon source…

Q: Your lab section performed the alcohol fermentation experiment as shown in the corresponding video.…

A: Alcohol by volume (abbreviated as ABV, ) is a standard measure of how much alcohol  is contained in…

Q: During cell couting, when the data are plotted in an ordinary graph or on a cross-section paper, why…

A: For experiments involving growth of cells whether bacterial or eukaryotic the results of a growth…

Q: Create a schematic diagram of the experimental procedure down below

A: Chlorophyll is the pigment contained in special membrane-enclosed organelles called "chloroplasts"…

Q: If I was interpreting the results of Durham tubes, Glucose, Lactose, Sucrose, for Staphylococcus…

A: The broth tubes also contain sugars other than glucose such as lactose and mannitol.  Due to a…

Q: Can you classify the following biochemical tests based on the following criteria and explain why you…

A: Different bacteria survives on different biochemical reactions. These biochemical reactions are used…

Q: Hello, I want you pleaseeee to make a dichotomous key based on provided experiments to identify a an…

A: The method of identifying an unknown bacterium is a precise one, requiring cautious perception and…

Q: a. Explain whether or not any of the methods in fi gure 2.9 could beused to determine the total…

A: Since, the question a. in incomplete as it requires figure 2.9 to be answered we will answer the…

Q: Question 4: Starting with a culture that contains 5.0 x 107 CFU/ml, describe a serial dilution…

A: Given values: Initial culture cell concentration = 5×107 CFU/ml Final colonies on the plate = 50

Q: Based on your observations recorded in the previous question, list the possible genotype(s) of…

A: β-Galactosidasetest detects the presence or absence of Galactosidase enzyme in the bacterial strain.…

Q: This is multipart for the SAME question so please answer ALL The data below are gram stains after…

A: The whole question is related to Gram staining procedure. It is a method of staining which is used…

Q: A microbiology student was given a mixed culture of two different gram positive bacteria species to…

A: In Microbiology, we grow bacteria on culture medium. Culture media contains specific mixture of…

Q: Provide the Principles and detailed procedures of the following tests for the "Color Reaction of…

A: Hopkins - cole test Principle -In this test , concentrated H2So4 cause dehydration of tryptophan…

Q: Match the biochemical test mediums to the chemical components for test reaction: OTSI TSI and SIM…

A: Biochemical test media are specialised growth mediums created to promote the growth and metabolic…

Q: Although triple-X human females typically have normal offspring, what kinds of gametes, with respect…

A: Introduction In humans, the X chromosome is one of two sex chromosomes, the other is the Y…

Q: Complete the following questions for your gram negative rod unknown organism (a) What test did you…

A: The gram-negative rod group is a diverse and extensive category of bacteria characterized by their…

Q: If you had cultures of B. subtilis and E. aerogenes and forgot to label them, which of the following…

A: Some bacteria produce indole by deamination of amino acid tryptophan and shows positive indole test…

Q: Which of the following is similar to a block of highly purified “jello-like” substance that is not…

A: Micropippete is laboratory equipment used to transfer small quantities of liquid. Restriction…

Q: Nitrate reduction test can be used to differentiate between Pseudomonads and Enterobacteriaceae. You…

A: The nitrate reduction test is used to differentiate between Pseudomonads and Enterobacteriaceae or…

Q: What would you need to culture the following microorganisms? (Note the hints and/or choices after…

A: Thermophile - Organisms tolerant to high temperature  Mesophile - organisms tolerant to moderate…

Q: Which of the following are positive for the production of indoor from the amino acid tryptophan?

A: indole is produced by reductive deamination from tryptophan through the intermediate molecule indole…

Q: Sulfur Indole Motility (SIM) Medium H2S produced, color and +/-: ______________________________…

A: Sulphur indole motility medium used to test the ability of am organism to have properties of…

Q: An Enteropluri test (including VP), as used in Practical 4, gave the following results code: 7 2 3 O…

A: Digestive enzymes are categorized primarily based totally on their goal substrates: Lipases break…

Q: In the amino acid decarboxylase test (lysine decarboxylase), we added mineral oil to the tube before…

A: Introduction The Lysine Decarboxylase Test Is Based On Some Bacteria's Capacity To Convert L-lysine…

Q: Lysine decarboxylase media is yellow after incubating an organism in it at 37 degrees for 24 hours.…

A: Lysine decarboxylase is an enzyme that is used in decarboxylation test. It is used to distinguish…

Q: Nicotiana tabacum cells are cultured to produce a polysaccharide gum. The reactor used is a…

A: The system or any device which provides a controlled environment for the growth of the…

Q: Which of the following is FALSE?   Question 3 options: A Some bacteria reduce nitrate to nitrite…

A: Biochemical tests are the basic tests that are used for bacterial identification. These tests help…

Q: A water sample from which 10, 1, and 0.1 ml/100mL dilutions resulted in 5, 4 and 3 positive…

A: Most probable number (MPN) is a method for estimation of the concentration of viable micro-organisms…

Q: Which of the the following results of the extracellular enzyme tests is FALSE?    Question 3…

A: Answer: Extracellular Enzymes or Exoenzymes are the enzymes secreted by the organisms or cells and…

Q: A relatively large zone of inhibition surrounding an antimicrobial disk on a Kirby-Bauer test plate…

A: The Kirby-Bauer test was developed by William Kirby, A. W. Bauer and their colleagues. It is also…

Q: Solid media is usually prepared with a 1-2% final concentration of agar. If these plates were…

A: Solid media is the type of media that is made up of liquid substances like distilled water and other…

Q: Which of the following is FALSE about the Nitrate Reductaion Test, procedure and interpretation?…

A: Among the many biochemical tests conducted on unknown bacterial isolates to identify them, nitrate…

Q: Using the date in the photo of the two syringes below, answer these six questions: QUESTIONS 1.…

A: The given data and the graph represent the relationship with the photosynthesis process. The…

Q: You perform the Methyl-Red(MR)& Voges-Proskauer(VP)test and gather the followingresults:…

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: Discuss the significance of the following processes and what constituent is being determined: a.…

A: Borntrager’s Test: This is a quantitative chemical test for the glycosides in any given sample, this…

Q: What will be the results of the following experiment with explanations: A. Gram-positive bacteria…

A: The response of Gram-positive and Gram-negative microscopic organisms is examined in this experiment…

Industrial Processes

Industrial processes result in the manufacturing of products (biological products, such as enzymes, medicine) on a large scale using different methods, such as physical and chemical procedures. Few products such as pharmaceuticals, agricultural products, food items, etc., involve microbes and their enzymes. Such processes are termed industrial biological processes. Microbiology and biotechnology enable us to utilize the ability of microorganisms to produce enzymes, primary metabolites, and secondary metabolites, which in turn help to produce the desired products.