Describe your color observations of the Nitrate test. a. after adding Reagents A, B (and/or znic): b. Did the organism reduce nitrate? (yes or no) c. Is the final product nitrate, nitrite or ammonia/nitrogen gas? Question 13 4 pts Based on your observations of the SIM test: a. Did sulfur reduction occur (yes/no)? b. Did the organism produce the enzyme tryptophanase (yes/no)? c. Is the organism positive or negative for the motility test?

You have several different media onto which you inoculated eight strains of yeast (A-H). The media include a rich medium, an unsupplemented minimal medium, and minimal media each supplemented with one vitamin. Of the yeast strains, one is a prototroph and seven are auxotrophs for a vitamin. After overnight incubation, the following results were observed (tan patches represent growth): D plate 1 (A) B DE F GH plate 5 plate 4 plate 6 Which plate contains an unsupplemented minimal medium? [Select] Which plate contains a rich medium? [Select] plate 2 Which strain is a prototroph? [Select] Strain E is an auxotroph for niacin. Which plate reveals this specific auxotrophy? [ Select] plate 3 plate 7 One strain is an auxotroph for both choline and pantothenic acid. Which one is this most likely to be? [Select]

QUESTION 4 Match the following regarding the Gram stain. A. last step in the procedure primary stain В. crystal violet counterstain C. nigrosin mordant D. 95% ethanol docolorizing agent E. methylene blue F. jodine

Please submit your assignment on the following topic 1. Lipid Hydrolysis Test principle and their importance? 2.Starch Hydrolysis Test principle and their importance? 3.Nitrate Reduction Test principle and their importance? 4.Voges Proskauer Test principle and their importance? 5.Methyl Red Test principle and their importance?