The gDNA sample that was isolated together with 12 other samples from other species were up for profiling. One of the target genes to be studied from these samples is the Nori gene. An optimized PCR protocol using a final concentration of 100 ng/μL gDNA as template can sufficiently amplify the gene in a PCR with a 25-µL reaction volume. Copy and complete the table below: Component PCR buffer MgCl2 dNTPs F. primer R. primer Taq pol gDNA temp sdH₂O Total Vol. Initial [] 10x 50 mM 10 mM 5 μM 5 μM 5U/μL 50 ng/μL Final [] 1x 1.5 MM 0.2 mM each 0.2 μΜ 0.2 μΜ 1U/rxn 20 ng/rxn --- Volume (μL) for 1x 25.0 μL Volume (µL) for (n+2) mastermix (____x)
Molecular Techniques
Molecular techniques are methods employed in molecular biology, genetics, biochemistry, and biophysics to manipulate and analyze nucleic acids (deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)), protein, and lipids. Techniques in molecular biology are employed to investigate the molecular basis for biological activity. These techniques are used to analyze cellular properties, structures, and chemical reactions, with a focus on how certain molecules regulate cellular reactions and growth.
DNA Fingerprinting and Gel Electrophoresis
The genetic makeup of living organisms is shown by a technique known as DNA fingerprinting. The difference is the satellite region of DNA is shown by this process. Alex Jeffreys has invented the process of DNA fingerprinting in 1985. Any biological samples such as blood, hair, saliva, semen can be used for DNA fingerprinting. DNA fingerprinting is also known as DNA profiling or molecular fingerprinting.
Molecular Markers
A known DNA sequence or gene sequence is present on a chromosome, and it is associated with a specific trait or character. It is mainly used as a genetic marker of the molecular marker. The first genetic map was done in a fruit fly, using genes as the first marker. In two categories, molecular markers are classified, classical marker and a DNA marker. A molecular marker is also known as a genetic marker.
DNA Sequencing
The most important feature of DNA (deoxyribonucleic acid) molecules are nucleotide sequences and the identification of genes and their activities. This the reason why scientists have been working to determine the sequences of pieces of DNA covered under the genomic field. The primary objective of the Human Genome Project was to determine the nucleotide sequence of the entire human nuclear genome. DNA sequencing selectively eliminates the introns leading to only exome sequencing that allows proteins coding.
helping tags: biochemistry, PCR, polymerase chain reaction, PCR cocktail preparation
Will upvote, just please help me solve the answers for the mastermix.
Please show solutions. Thanks.
![The gDNA sample that was isolated together with 12 other samples from other species were up for
profiling. One of the target genes to be studied from these samples is the Nori gene. An optimized PCR
protocol using a final concentration of 100 ng/µl 8DNA as template can sufficiently amplify the gene in a
PCR with a 25-µl reaction volume. Copy and complete the table below:
Initial [ ]
Final [ ]
Volume (µL) for
Volume (uL)for
(n+2)mastermix
Component
1x
PCR buffer
10x
1x
MgCl2
50 mM
1.5 mM
DNTPS
10 mM
0.2 mM each
F. primer
5 μΜ
0.2 μΜ
R. primer
5 μΜ
0.2 µM
Таq pol
5U/ul
10/rxn
9DNA temp
50 ng/ul
20 ng/rxn
sdH20
Total Vol.
25.0 µl](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F0a7a77fd-30b4-4c19-b3e9-8b3a92ea531c%2Fc74fffcc-6aaa-4be6-91dd-ad7ed05ad0cd%2Fn62al2u_processed.png&w=3840&q=75)
Trending now
This is a popular solution!
Step by step
Solved in 4 steps with 2 images
