All of the following are needed for PCR except: A) dNTP nucleotide monomers B) Oligonucleotide primers C) Restriction endonuclease D) Template DNA E) DNA polymerase

Explain why a positive control and negative control are included in PCR experiments. ​Explain the three steps involved in each cycle of polymerase chain reaction.Why is loading dye added to the DNA sample for gel electrophoresis? ​Explain the function of the following components in a PCR reaction:− Primer, dNTP, MgCl, Taq polymerase, buffer.

You have ligated a foreign DNA fragment into the EcoRI site in multiple cloning site of pUCI18. You then transform how E.coli with the ligation mixture. Some of the bacterial colonies growing on the nutrient agar plate that contains ampicillin and Xgal are white and some are blue. Bacterial colonies that are white a) contain recombinant plasmid DNA b) have hydrolyzed ampicillin to form a colored compound c) are untransformed bacteria d) contain non recombinant plasmid DNA

A) replicative B) prime C) conjugative D) Hfr