Q: Briefly describe the procedure of AluQuant human DNA quantitation system. Explain why it is…
A: The AluQuant system used for human deoxyribonucleic acid quantitation uses certain probes that help…
Q: Describe several applications of DNA fi ngerprinting and microarray analysis.
A: DNA fingerprinting: - It is a method which provide genetic information of any living thing. It…
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A: DNA extraction is the procedure of isolating the Deoxyribonucleic acid from biological samples. PCR…
Q: What are STRs, and why are they useful for DNA profiling?
A: Introduction In the genome there are usually two type of nucleic acid sequence present. One is Non…
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A: NGS is an advanced DNA sequencing technique used high throughput and massively parallel sequencing…
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A: Deoxyribonucleic acid or DNA is a polymer composed of two polynucleotide chains that coil around…
Q: In assessing extracted DNA quality, what does A280 indicate? aromatic amino acids nitrogenous bases…
A: DNA is extracted from specific cells to study the extracted DNA. The purity of extracted DNA is…
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A: Ribosome profiling- it is carried out on a split sample, with parallel libraries constructed for…
Q: Which of these is not a tool for comparing DNA sequences? PLINK Fasta BLAST A dotplot e.g. dotlet
A: DNA polymerase is an enzyme. A primer is a single-stranded DNA fragment that binds to template DNA…
Q: describe the principles and procedures used with CODIS for DNA -based identification
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Q: From your knowledge about DNA microarray, answer the following: Mention the name and the color of…
A: DNA microarray is basically a collection of microscopic DNA spots that are attached to the solid…
Q: Describe a method of labeling a DNA probe. Explain how this probe maybe detected after DNA…
A: The probe is basically a single stranded sequence of DNA or RNA which is used commonly for the…
Q: Why the DNA sequencing alone is often not sufficient to produce a genome assembly of desired…
A: DNA sequencing is basically the process by which the nucleic acid sequence can be determined. the…
Q: _________ Techniques Permit Detection of Specific DNA Fragments and mRNAs.
A: Gel electrophoresis is a method used to segregate the DNA fragments depending on their size and…
Q: t tandem repeats) multiplexing combined with PCR requires a relatively large volume of high quality…
A: Short tandem repeats are a small sequence of nucleotides containing repetitive nucleotides ranging…
Q: Sanger sequencing Write a precise and accurate differential report on the above sequencing…
A: DNA sequencing is the process of determining the sequence of nucleotides (As, Ts, Cs, and Gs) in a…
Q: Discuss the use of gel electrophoresis for the separation of macromolecules (DNA, RNA and protein)…
A: Gel Electrophoresis It is a technique which is used to separate the fragment of macromolecules like…
Q: Amazingly, the sensitivity of STR profiling requires only ___________ DNA-bearing cells to obtain an…
A: Deoxyribonucleic acid or DNA is the hereditary material that is present in all organisms. DNA…
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A: PCR - (polymerase chain reaction) is used to amplify the DNA sequence through repetitive cycles. It…
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A: DNA is genetic material in almost all living organism that transit from one generation to another
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A: Because plasmid is a circular DNA molecule, this is the case. It becomes linear but not fragmented…
Q: Which best desrcibes a 'CRISPR'? a delivery vector a small DNA binding protein a…
A: The correct option is - clusters of short palindromic DNA .
Q: what is the Jusage of DNA separation in gel electrophoresis
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A: The Sanger sequencing is also known as the chain termination method, it is a method used to…
Q: Explain the use of a probe in screening a DNA library
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A: The tiny living creatures such as bacteria, viruses, fungus, algae, and protozoa have a significant…
Q: In biotechnology procedures, a(n) ____ is a nucleic acid fragment that is used to search for and…
A: In biotechnology procedures, a ___ is a nucleic acid fragment that is used to search for and…
Q: Recombinant DNA techniques that require gel electrophoresis
A: Following are the applications of gel electrophoresis in DNA recombinant technology.
Q: Restriction enzymes and DNA ligase are key components when preparing cloning vectors. True or False.
A: Main components for preparation of cloning vector are : Origin of replication Marker gene…
Q: PCR is a powerful technique to screen and amplify segments of DNA for use in recombinant protein…
A: Introduction PCR is a method for amplifying a specific region of DNA from a mixed template. Reagents…
Q: Microarray hybridization is used mostly in transcript profiling or assaying DNA variation. Although…
A: A usual microarray technology includes the hybridization of an mRNA with its original template of…
Q: An individual’s unique set of_______ can be used in DNA profiling. a. DNA sequences c. SNPs b. short…
A: Introduction In the genome, there is usually two types of nucleic acid sequence present. One is…
Q: The blotting technique used toidentify the isolated protein is ________A. Northern blottingB.…
A: Proteins are the type of macromolecules that are synthesized in the cytoplasm during the process of…
Q: Ruwaifa want to compares a nucleotide query sequence what option he will opt in BLAST and why?
A: BLAST is Basic local alignment search tool used to compare the queries in a DNA database. They are…
Q: write the pros and cons of crispr technology in bullet points with explaintion
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Q: Explain why the 16S rRNA gene sequencing is suitable for bacterial identification in general and why…
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Q: The current state-of-the-art in forensic DNA profiling involves the PCR-amplification and analysis…
A: PCR is a process in which millions of copies of a specific sequence of DNA can be made in a matter…
Q: Aside from gel electrophoresis Give another method to quantify DNA. Explain the concept behind this…
A: DNA quantification is a type of quantification of nucleic acids that is used for the determination…
Q: In assessing extracted DNA quality, what does A230 indicate? nucleic acids background reading…
A: DNA and RNA were characterized using several methods for assessing quantity, quality, and molecular…
Q: Short tandem repeats (STRS) in DNA are often used in forensic analysis and for determining…
A: Short tandem repeats (STRs) are short repeated successions of DNA (2-6 bp) that record for roughly…
Q: give the significance/role/effect of the reagent/condition in the isolation or analysis of a…
A: DNA isolation is a process of isolation of DNA from biological sample like body fluid, tissue, etc.…
Q: Describe several applications of DNA profiling and microarrayanalysis.
A: Deoxy ribonucleic acid (DNA) is the genetic material of most organisms that carry coded genetic…
Q: To achieve a 10x depth coverage for a genome of length 10 Mbp, how many reads of 100 bp are…
A: A Coverage is multiplier based on total size of the genome. Formula = Coverage = (read length) x…
Q: Some recombinant DNA techniques depend on the specific hybridization (or annealing) between two…
A: Recombinant DNA technology comprises altering genetic material outside an organism to obtain…
Q: Index sequences that are small segments of DNA with sequences that are unique and not found anywhere…
A: The DNA is the genetic material in most of the organisms. it is located within the nucleus of the…
Q: Describe two different types of protein microarrays, and discuss their uses.
A: Genomics and proteomics are relatively new branches of genetics. Genomics is the study of all the…
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- Question 4. What is the probability that a restriction enzyme will cut DNA if the recognition sequence for the enzyme is 5'-GGATCC-3' ? b) Assuming that the % GC is = 60%. O (2/10)4 (3/10)² O (3/10)4 (2/10)² O (1/6)4 O (1/4)6 O (6/10)4 (4/10)2Question 4. What is the probability that a restriction enzyme will cut DNA if the recognition sequence for the enzyme is 5'-GGATCC-3'? a) Assuming that the four bases are equally likely to be found in a DNA molecule. O (1/4)6 O (1/4)4 O (1/6)4 O 1 in 6 O (1/6)6| Choose ) [ Choose] metagenomics whole-genome shotgun approach bioinformatics ed GenBank genomics
- Question -The FDA has authorized the use of direct-to-consumer testing for three mutations in BRCA genes that elevate cancer risk, but cautions that a negative result does not rule out increased cancer risk. How can this be true? A. It is impossible to trust companies that are selling genetic tests. B. They have a conflict of interest, and so the tests should be used for entertainment value only C. These tests are not highly accurate, and false negatives are possible. Individuals with a family history should have a negative result confirmed with a different test to be sure they are truly at low risk of developing cancer There are more ways to get cancer than a mutation in the BRCA gene. D. The test only detects three out of more than 1,000 known BRCA mutations. This means a negative result does not rule out the possibility that an individual carries other BRCA mutations that increase cancer risk..QUESTION 7 Primers are needed to start a PCR reaction True O False QUESTION 8 Restriction enzymes specifically recognize and cut short sequences of DNA called introns. O exons. O sticky ends. restriction sites. QUESTION 9 The DNA profiles used as evidence in a murder trial look something like supermarket bar codes. The pattern of bars in a DNA profile shows O the order of genes along particular chromosomes O the order of bases in a particular gene O the presence of differently-sized fragments of DNA O the presence of dominant or recessive alleles for particular traits O the number of chromosomes and whether any are damagedQUESTION 5 Match the vocabulary word with the proper definition: enzyme that joins two pieces of DNA ✓ first human protein to be produced by genetic engineering 00000 ✓ process that makes many copies of a gene or other DNA segment the process of isolating and making copies of a gene ✓ the process of placing recombinant DNA into a living cell circular DNA that is not part of a chromosome V genetically modified plants changing an organism by transforming with recombinant DNA DNA ligase II. recombinant DNA III. transgenic crop IV. polymerase chain reaction V. gene cloning VI. genetic engineering VII. transformation VIII. plasmid IX. biotechnology X. insulin the use of technology to change the genetic makeup of living things for human purposes made by joining DNA from two different
- b) Among the progeny from the Hfr3 mating above, you find one that has the genotype: Abc de F Draw out the gene transfer and crossover(s) that produced this outcome: c) Among the progeny from the Hfr3 mating above, you find one that has the genotype: A B c d e f Draw out the gene transfer and crossover(s) that produced this outcome:Question 2. You have a wild-type strain of E. coli with the genotype A B C D EF You introduce an F+ plasmid into your wild-type strain and isolated a few Hfr derivative strains that you call Hfr1, Hfr2, and Hfr3. You are studying several new genes in E. coli with interesting phenotypes. You obtain a multiply mutant strain with chromosomal genotype: a b c d e f a) You mate each Hfr strain to your multiply-mutant strain in a separate experiment. At various times you interrupt the matings and plate the bacteria under conditions in which only the recipient strain can grow. You obtain the following earliest-time-of-entry data, in minutes: Gene Hfr1 Hfr2 Hfr3 A B C D E F 11 13 7 I 26 16 11 9 5 31 15 27 31 11 Draw a map of these genes that is consistent with the data, including all the genes and Hfr origins, the distances between them (in minutes), and the direction of transfer of each Hfr.QUESTION 2 Which of the following statements about nucleic acid synthesizing enzymes (polymerases) is FALSE? O A. DNA pol I can use DNA as a primer, DNA pol III can use RNA as a primer. O B. The DNA polymerase III holoenzyme is highly processive, DNA polymerase I has low processivity. OC. The E. coli polymerase III core enzyme has both DNA synthesis and proofreading activity. O D. E. coli polymerase I consists of one long polypeptide chain that has three separate activities, polymerization, proofreading, exonuclease. O E. The Klenow enzyme has the ability to remove the primers that begin each Okazaki fragment.
- Question 3 Choose the false statement below. O CRISPR is a protein complex which causes the destruction of specific sequences of nucleic acids; this strategy serves as a natural intracellular defense system against any incoming genetic material. O Although only recently developed technology, CRISPR is already being used in the medical industry to deliver protein or RNAS for vaccination. O CRISPR has now been harnessed by scientists to more easily genetically modify eukaryotic organisms even in their adult multicellular form.Question 1. Restriction endonucleases can be isolated from a number of bacteria. In bacteria restriction endonucleases. a-restrict chromosomal DNA that is heavily mutated b-restrict chromosomal DNA with significant regions that have been Deleted. c-restrict the DNA of invading bacteriophages. d- all of the above Question 2. In a PCR reaction the step in which DNA polymerase replicates the DNA is referred to as a- denaturation b- annealing C- extension d- initiationQUESTION 4 After you get your gene block, you do a restriction enzyme digest and ligation reaction with the plasmid/gene, so you can proceed to bacterial transformation! How does the plasmid get into the bacteria though? O You mix the plasmid in lipids with several other plasmids to make lentiviral vector that'll infect all the other cells. O The plasmid will be transmitted through a pilus to the other bacteria. O You make the bacteria chemically competent using calcium chloride, and then subject the cells to heat shock O Bacteria will naturally take up the plasmid so you don't need to worry about it.