Match the vocabulary word with the proper definition: enzyme that joins two pieces of DNA ✓ first human protein to be produced by genetic engineering 1. DNA ligase II. recombinant DNA III. transgenic crop ✓ process that makes many copies of a gene IV. polymerase chain reaction or other DNA segment V the process of isolating and making copies of a gene V the process of placing recombinant DNA into a living cell circular DNA that is not part of a chromosome genetically modified plants ✓ changing an organism by transforming with recombinant DNA V. gene cloning VI. genetic engineering VII. transformation VIII. plasmid IX. biotechnology X. insulin the use of technology to change the genetic makeup of living things for human purposes made by joining DNA from two different

You want to amplify a specific region of DNA during PCR. Your primers should   a Both should be an exact replica of the beginning of the specific gene you want to amplify b Both should be complementary to the beginning of the specific gene you want to amplify c One primer should be identical to the beginning of the gene sequence while the other is identical to the end of the gene sequence d One primer should be complementary to the beginning of the gene sequence while the other is complementary to the end of the gene sequence e One primer should be identical to the beginning of the gene sequence while the other is complementary to the end of the gene sequence

Generate a concept map that includes all the specifics below: • Classification based on: 1. their effect on the DNA 2. on their phenotypic effect. . Kinds of DNA damage and lesions (spontaneous and induced) produced by specific exposures. (Note: don't forget transposons and CRISPR Cas9) Specific repair mechanisms that fix each kind of DNA damage or lesion (during/post replication). • Include information about the number of replication cycles for a dominant mutation to cause a phenotype. And the number of cycles for a recessive mutation to possibly cause a phenotype. (Hint: don't forget to think about each strand of both parental copies of each chromosome) O

Choose the single most appropriate description of how most Next Generation sequencing methods work.   Template DNA is attached to 'chips', free di-nucleotides are added to synthesise new DNA, computers read the results.   Template DNA is attached to 'chips', di-deoxy nucleotides are added to synthesise new DNA, computers read the results.   Sample DNA is broken into short fragments and synthetic oligonucleotides are added to them. They are bound to a membrane and sequenced using lasers.   Sample DNA is broken into short fragments and synthetic oligonucleotides are added to them. The fragments are then bound to a membrane and sequenced using light emission as the reporter.   Sequencing by synthesis of short template fragments, using various 'reporters' and computing power to rebuild lengthy sequence data in silico using reference genomes.   Sanger sequencing of short fragments, using various 'reporters' and computing power to…