what is the Jusage of DNA separation in gel electrophoresis

Calculate the purity of the plasmid DNA. Do you think the plasmid is pure? Why?

Use a drawing to illustrate the principle of DNA gel electrophoresis. Indicate roughly the comparative electrophoretic mobilities of DNAs with 150, 600, and 1200 bp.

Which of the following gels is the preferred choice for separation of DNA fragments? Agarose Polyacrylamide SDS PAGE (sodium dodecyl sulphate polyacrylamide gel electrophoresis) Agar

describe the two main parts of a plasmid DNA extraction and purification procedure including the purpose of each step

What are the characteristics of a high-quality DNA extract? What do you do to determine the quality of your DNA extracts?

Elaborate on the basic principle you would adopt to isolate an amplified DNA fragment using agarose gel during electrophoresis. In you answer, provide factors that would facilitate migrationof the DNA fragment though the gel.

you have transformed E.coli cells with a plasmid containing the gene for the enzyme beta-lactamase you have used 3µl of a stock solution of 2.5ng/µl DNA. You transformed 150/µl of competent cells, using the calcium chloride method. you have added 600µl of luria broth to the cells and then plated out 200µl on triplicate plates. upon counting the colonies, you got the following results: plate 1: 120 cfu plate 2: 35 cfu plate 3: 95 cfu please answer the following question: 1. what new characteristic will the cells have ater transformation? 2. how are you going to test for the new characteristic? 3. calculate the transformation efficiency of the experiment?

Why do longer DNA molecules move more slowlythan shorter ones during electrophoresis?

What are the steps in extracting DNA from a Banana using simple household materials like dishwashing liquid and cold isopropyl alcohol? Explain the principles