QUESTION: Microbes from a blood culture inoculated with blood drawn from a catheter were determined by the appearance of growth on Mannitol Salts Agar and through other tests to be Staphylococcus epidermidis. It can be concluded that: a.  a bloodstream infection caused by S. epidermidis is occurring in the patient from whom the blood was drawn b.  The bacteria fermented mannitol, changing the color of the growth medium from pink to yellow. c  S. epidermidis is present in the sample as a normal flora contaminant d.  That in order to determine if a bloodstream infection is present, these results must be compared to a blood culture of a sample drawn from a peripheral vein

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Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.