Case Study: You are asked to inoculate lactose media (broth) with E. coli that was growing exponentially in glucose media (broth). You monitor the growth of these cells in the lactose broth using a viable plate count technique and plot the Growth Curve for E. coli growing on lactose. After a day of monitoring the cells, you note that the culture entered into lag phase growth initially and is now currently growing exponentially. In addition, you find that the cultures doubling time is 30 minutes. You know that the doubling time of E. coli in glucose media is 20 minutes. You did not change the oxygen content or temperature at which you were growing the cells, E. coli is still aerobically respiring at 37°c. Question: If you want to grow E. coli as quick as possible would you grow it on lactose or glucose? O Mixing the two carbon sources would provide the most optimal growth conditions. O Lactose because of the generation time being faster. O Lactose and an anaerobic environment. O Glucose because of the generation time being quicker.

Answer all the questions. Draw the diagram by hand:

Hello, please answer the following Microbiology question correctly and fully. Please explain your answer. Question: How many hours minimum must you incubate the gelatin hydrolysis test?  *If you correctly answer the question correctly, I will provide a Thumbs Up to you. Thank you.

Using the data in the picture below, answer the five questions in the pictures.

Please help me with these two questions. Thank you so much.

Catalase: 1. Explain the incubation conditions 2. Explain the reagents being added 3. Explain the observations in Catalase 4. Explain the interpretations in Catalase

A culture of S. cerevisea has an overnight OD of 2.3 (1.0 OD is approx 1.0x107 cells/ml) You will be plating 100µl onto agar and want the final count of colonies on the plate to be around 300 colonies.  How much of the 2.3 OD culture must you use to get a 500µl subdilution (with sterile water), so that you have diluted enough to get approx 300 colonies per 100ul

Develop a spreadsheet to predict as functions of time with concentration, volimme and permeate flows for a batch ultrafiltration. Assume that the flux depends on concentration as follows: J(L/M²/H) = A*EXP(-bC) where C is the retentate concentration in g/l. Assume that the recirculation rate is kept high enough that shear rate dependence is eliminated. Do a sample run with C.= 0.5 g/l, Cfinal = 50 g/l, A = 50, and b = 0.02. Assume a filtration area of 19.4M² and an initial volume of 3255 L. Assume σ (protein) = 1.0 or σ (protein) = 0.95 or σ (protein) = 0.90.

Based on the growth curve, which ONE of the following best describes what would happen if sample of culture in the presence of drug B is inoculated onto drug-free growth medium?   Select one: A.   Growth present, but cell count increased by at least 99.9% of the original culture   B. No growth after 24 hrs C. Growth at 24 hrs D. Not possible to predict because the culture could either be alive or dead E. Growth present, but cell count reduced by at least 99.9% of the original culture

Please answer fast Dilution Problem. A culture of Staphylococcus is diluted as follows: (1) 20mL are added to 80mL of water. (2) 10uL from (1) are then added to 9.99mL of water. (3) A 10-2 dilution is made from tube # (2). (4) 100uL from (3) are plated for a pour plate and incubated.   Growth Problem.A culture with approximately 2x103 cells/mL were incubated. After 3 hours, the number of cells had increased to 3x105. a) How long was the generation time in minutes?b) How many generations have occurred