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what is the prognosis and diagnosis of a renal cell carcinoma patient using H and E stain immnophenotyping and Qpcr.
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- Discusses the methods to -1 optimise fluorescent cellular (staining (priority first List the freezing -2 parameters of the slow cooling technique Parameter of verification -3 precedure of freezing 9There are 2 main ways of treating tumours - external irradiation by radiotherapy and internal radiopharmaceuticals. Using the points shown below, describe each process and compare and explain the advantages and disadvantages of using radiopharmaceuticals as opposed to external irradiation. An example of a specific condition can be used to illustrate the points. area of treatment required position of tumour ionising range precautions needed after treatmentGive typing answer with explanation and conclusion 8. Considering the principle of the Neutral Red bioassay, describe, in details, the expected appearance of the cheek/buccal cells if exposed to a toxic substance such as cigarette ash or possibly any other type of toxic substance.
- Question:- Starting with data collection, describe the steps necessary to determine a 3-Dimensional structure using electron microscopy methods.WIDAL TEST Principle: The test depends on the ability of antibody in the patient’s serum to agglutinate the stained bacterial antigens. When this occurs, the aggregates become clearly visible to the naked eye. Materials: Test tube 75 x 12 mm Physiological saline (0.9%) Incubator or water bath Slides Procedure: A. Rapid Slide Test Semi-Quantitative Method 1. Using a graduated pipette add the following amounts of serum to consecutive circles on a slide for each dilution under test. 0.08 ml, 0.04 ml, 0.02 ml, 0.01 ml and 0.005 ml 2. Thoroughly resuspend the antigen and add a drop to the appropriate circle on the slide. 3. Mix the drops and spread to cover the entire test circle. 4. Gently and evenly, rock and rotate the test slide for 1 minute then examine the slide for agglutination. 5. Results obtained correspond to tube agglutination titer of 1:20, 1:40, 1:80, 1:160, 1:320 respectively. 6. It is advisable to confirm a slide…Briefly discuss 2 advantages and 2 disadvantages of using the paraffin embedding method for histological examination of tissues as opposed to the frozen technique
- Topic: Isolation of Crude Ovalbumin from Egg White by Ammonium Sulfate Precipitation (Salting Out) Egg whites contain about 88.5% water and about 10% proteins, from which ovalbumin is the major component. The eggs were cracked open, and the egg whites were separated carefully from the yolk. Stirring of the egg whites was done to break up the membrane. This was followed by filtration through cheesecloth, where a clear filtrate was obtained. About 40.0 mL of the filtered undiluted egg white was measured, and 0.10 mL of 1 M acetic acid was slowly added for every 1.0 mL of egg white with constant stirring. A turbid mixture with few amounts of white precipitate may be expected to form. These may be removed by filtering the mixture using a cheesecloth. What is the significance of adding 1 M acetic acid to the filtered egg white?Topic: Isolation of Crude Ovalbumin from Egg White by Ammonium Sulfate Precipitation(Salting Out) Egg whites contain about 88.5% water and about 10% proteins, from which ovalbumin is the major component. The eggs were cracked open, and the egg whites were separated carefully from the yolk. Stirring of the egg whites was done to break up the membrane. This was followed by filtration through cheesecloth, where a clear filtrate was obtained. About 40.0 mL of the filtered undiluted egg white was measured, and 0.10 mL of 1 M acetic acid was slowly added for every 1.0 mL of egg white with constant stirring. A turbid mixture with few amounts of white precipitate may be expected to form. These may be removed by filtering the mixture using a cheesecloth After filtration, 30.0 mL of the filtrate was obtained, and the solution was brought from 0% to 40% saturation by adding the required amount of powdered ammonium sulfate, (NH4)2SO4 After adding ammonium sulfate, the mixture was allowed to stand…QUESTION:- Develop a test where IgG is characterized based on its physical, chemical, or biological properties.
- INTERPRETATION OF RESULIS NEGATIVE: Two lines appear. One colored line should be in the control region (C), and another apparent colored or faded color line adjacent should be in the test region (T). This negative result indicates that the drug concentration is below the detectable level. POSITIVE: One colored line appears in the control region (C). No line appears in the test region (T). This positive result indicates that the drug concentration is above the detectable level. INVALID: Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test using a new test panel. If the problem persists, discontinue using the lot immediately and contact your local distrībutor. READING OF RESULTS INTERPRETATION SONTROL TEST SAMPLEThis is homework not a test! From NTSA case study https://static.nsta.org/case_study_docs/case_studies/cystic_fibrosis.pdf Please help with questions 2, 3 and 4 of part four 2. "The successful use of gene therapy to cure SCID syndrome (2000) is hoped to be a permanent cure for those patients because a good copy of the problem gene was inserted into the patients' blood stem cells in the bone marrow (hematopoietic stem cells). Once white blood cells enter the blood stream they have a limited life span, on the order of a few week to months. The blood stem cells are the cells that create more white blood cells to replace those that are lost. If the gene was only inserted into the circulating mature white blood cells, the patient would only be temporarily cured until those cells were used up or died." The current gene therapy approaches to cure CF involve inserting a functional CFTR gene into the mature epithelial cells of the lungs. In light of the preceding paragraph, do you think that…Question: A rapid COVID antigen test: may result in a false positive if you test too early. detects antibodies made against the virus in the body. can detect the COVID and flu virus simultaneously in one strip. may result in a false negative if you test too early.
