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Question: In order to determine the doubling time of E. coli a viable plate countris performed. This means that you are truly counting O Dead cells only via a microscopic count O Live cells only via membrane filtration or serial dilution O All cells via membrane filtration or serial dilution

Question: In order to determine the doubling time of E. coli a viable plate countris performed. This means that you are truly counting O Dead cells only via a microscopic count O Live cells only via membrane filtration or serial dilution O All cells via membrane filtration or serial dilution

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Case Study: You are asked to inoculate lactose media (broth) with E. coli that was growing exponentially in glucose media (broth). You monitor the growth of these cells in the lactose broth using a viable plate count technique and plot the Growth Curve for E. coli growing on lactose. After a day of monitoring the cells, you note that the culture entered into lag phase growth initially and is now currently growing exponentially. In addition, you find that the cultures doubling time is 30 minutes. You know that the doubling time of E. coli in glucose media is 20 minutes. You did not change the oxygen content or temperature at which you were growing the cells, E. coli is still aerobically respiring at 37°C. Question: In order to determine the doubling time of E. coli a viable plate countris performed. This means that you are truly counting O Dead cells only via a microscopic count O Live cells only via membrane filtration or serial dilution O All cells via membrane filtration or serial dilution O Those cells that can be seen with dye
Transcribed Image Text:Case Study: You are asked to inoculate lactose media (broth) with E. coli that was growing exponentially in glucose media (broth). You monitor the growth of these cells in the lactose broth using a viable plate count technique and plot the Growth Curve for E. coli growing on lactose. After a day of monitoring the cells, you note that the culture entered into lag phase growth initially and is now currently growing exponentially. In addition, you find that the cultures doubling time is 30 minutes. You know that the doubling time of E. coli in glucose media is 20 minutes. You did not change the oxygen content or temperature at which you were growing the cells, E. coli is still aerobically respiring at 37°C. Question: In order to determine the doubling time of E. coli a viable plate countris performed. This means that you are truly counting O Dead cells only via a microscopic count O Live cells only via membrane filtration or serial dilution O All cells via membrane filtration or serial dilution O Those cells that can be seen with dye
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