Question: In order to determine the doubling time of E. coli a viable plate countris performed. This means that you are truly counting O Dead cells only via a microscopic count O Live cells only via membrane filtration or serial dilution O All cells via membrane filtration or serial dilution

Activity 13 Urine Culture Inoculating Urine with a calibrated loop   The number of microorganisms per milliliter recovered on urine culture can aid in the differential diagnosis of urinary tract infection (UTI). Plastic or wire inoculating loop available commercially, have been calibrated to deliver a known volume of liquid when handled correctly, thus enabling the microbiologist to estimate numbers of organism in the original specimen based on colony forming unit (CFU) of growth on cultures.   PROCEDURE: 1st day 1. Gently swirl the specimen bottle to mix the urine specimen. 2. Label all plated media with name of patients, clinical specimen used and date. Label at the bottom of plates and not on the cover. 3. Obtain a disposable calibrated loop. 4. Dip the loop straight into the urine specimen so that the loop part is completely covered. Withdraw straight out. See fig. 16-18 5. Inoculate blood agar plate (BAP) as shown in fig.16-19 6. Incubate at 35 – 37˚C for 18 – 24 hours. 7. Dispose…

Develop a spreadsheet to predict as functions of time with concentration, volimme and permeate flows for a batch ultrafiltration. Assume that the flux depends on concentration as follows: J(L/M²/H) = A*EXP(-bC) where C is the retentate concentration in g/l. Assume that the recirculation rate is kept high enough that shear rate dependence is eliminated. Do a sample run with C.= 0.5 g/l, Cfinal = 50 g/l, A = 50, and b = 0.02. Assume a filtration area of 19.4M² and an initial volume of 3255 L. Assume σ (protein) = 1.0 or σ (protein) = 0.95 or σ (protein) = 0.90.

Tube # 2c Table 3-Determination of the optimum temperature of catechol oxidase enzyme. 1st Absorbance 0 min. at Expt. Temp. 3c 4c Q12) After 10 min at experimental temperature was enzyme activity high or low at 75°C and 100°C? Support your answer by looking at the difference between 2nd and 1st absorbances. ( 5c Q13) Was the enzyme denatured at 75°C and/or 100°C% Support your answer by looking at what happens after returning enzyme to room temperature AFTER enzyme was subjected to experimental temperatures of 20 °C,40 °C,60 °C,80 °C and 100°C. "¹ /1 Q14) What happens to the enzyme structure and function when it becomes denatured? ( Potato Expt. extract Temp. (mL) 2 2 2 2 room temp 20 °C 40 °C 75 °C Boiling 100 °C dH₂O Catechol (mL) (mL) 0 0 0 0 13 || 13 13 13 Start Time: 4:20 Absorbance: 0.072 Start Time: 4:25 Absorbance: 0.114 Start Time: 4:25 Absorbance: 0.132 Start Time: 4:27 Absorbance: 0.097 2nd Absorbance after 10 min. in Expt Temp. Time for reading: 4:30 Absorbance: 0.128 Time…

0 O O Catalase is an enzyme responsible to toxify: H₂O2 toxify; H₂O detoxify; H₂O2 detoxify; H₂O from _product?

how did you get 104 as the dilution factor? I got 10-2 as the dilution factor for the CNFL plate

You counted 4, 6, 12, 3 cells in each of the 4.outed squares of a hemacytomeyer. What are the cells per milliliter in that culture? If you resuspended your cell pellet 2.5 mL, what is the total cell count? How many uL do you need to add to a new culture if you want 4250 cells?