A) How many origins of replication do you think the viral genome has, and why do you think this? B) Do your data support unidirectional or bidirectional replication of the viral genome? How have you come to this conclusion?
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- Question 2. What does DNA polymerase need in order to make contact with a replication origin?Question 9. What basal transcription factors can only bind to the DNA within the pre-replication complex after tata binding protein (TBP) bends the DNA?Question 3. How is the primer different in eukaryotic DNA replication than prokaryotic DNA replication?
- Question 4. Why is a single-stranded, circular DNA an ineffective template for DNA polymerase?There are 2 parts to this question: The following DNA strand (below) is about to undergo DNA replication. a) Please replicate the parental strands into two exact copies TC GATATCGG AGCTATAGCC b) place a centromere between the two replicated copies (or tell me where the centromere would be located),to 4 minutes) The schematic diagram below shows organization of the DNA replication fork. Match parts of the diagram (labeled A-F) with the corresponding term from the answer list (designated 31 parental duplex 5' 3' fork progression v A 1Lagging strand 2. An Okazaki tragment 3.Site of action of DNA topoisomerase 4 Leading strand 5. Site of action of DNA helicCase 6.Site of action of DNA ligase
- Answer the following questions with regards to DNA replication:1. In what direction does DNA polymerase read the parent strand?2. Describe why Okazaki fragments are formed (1-2 sentences)3. In no more than one sentence describe the function of the following enzymes as it pertains to DNA replication in E. coli : DNA polymerase III, primase and ligasePage 1 of 3 ZOOM DNA Replication_Protein Synthesis_Mutation Assignment *Please type your answers. 1. DNA synthesis and protein synthesis are two processes that are necessary for the cell. Why are these two processes necessary for the cell? How are they connected to each other? wious 4+ 144Replication of a circular DNA molecule can occur by either theta () replication or by rolling circle replication. Describe or explain three differences between these two modes of DNA replication (you must accurately describe differences between the two approaches to replication - an accurate statement about one form of replication that does not clearly indicate how the other form differs is not a correct)
- .. CTGATTCCGAA TG5 ACTIVITY 7.3.1 Given a part of DNA undergoing replication. Copy and write the corresponding bases in the new strands. Put an arrowhead in the appropriate end of each new strand to illustrate the direction of growth. .GACTAAGGCTTAG . CTGATICCGAATGS 5'... NEW STRANDS (Fill in the correct bases and supply arrowheads.)Provide a detailed description and hand-drawn figure for each of the following. (1) DNA Unwinding/Supercoiling (2) Replication Initiation (3) Replication Elongation (Replication Fork) (4) Replication TerminationWhich of the following are differences between prokaryotic DNA replication and eukaryotic DNA replication? (select all that apply) Group of answer choices: 1. Eukaryotic DNA is linear, while prokaryotic DNA is circular and undergoes theta replication 2. Eukaryotic genomes often have multiple chromosomes, while prokaryotic genomes are usually only made up of one chromosome 3. DNA polymerase 3 adds dNTPs to the growing DNA strand in eukaryotes, while DNA polymerase 2 adds dNTPs to the growing DNA strand in prokaryotes 4. In eukaryotes, DNA polymerase requires a primer to add nucleotides to growing DNA strand, while in prokaryotes, no primer is needed to add nucleotides to the growing DNA strand 5. Longer telomeres are added to the end of prokaryotic chromosomes than are added to the ends of eukaryotic chromosomes 6. Eukaryotic DNA will have multiple origins of replication, while prokaryotic DNA only have one