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A) How many origins of replication do you think the viral genome has, and why do you think this? B) Do your data support unidirectional or bidirectional replication of the viral genome? How have you come to this conclusion?

A) How many origins of replication do you think the viral genome has, and why do you think this? B) Do your data support unidirectional or bidirectional replication of the viral genome? How have you come to this conclusion?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Question 2. For your senior research, you end up studying the life cycle of an animal virus whose genome consists of a single circular, double-stranded DNA molecule. Your project is to define the number and location of the origin(s) of replication and to determine whether replication proceeds in one (unidirectional) or both (bidirectional) directions away from an origin. To accomplish this, you isolate many identical strands of DNA that have already been partially replicated. You cleave each piece of DNA exactly once with a restriction enzyme. You then observe the cut pieces of DNA using an electron microscope. Below is a schematic representation of what you observe. Remember that each line represents a different piece of DNA, and not a fragment of a larger piece. Using this data, answer the following questions: A) How many origins of replication do you think the viral genome has, and why do you think this? 30: F2 #3 *** E D 80 F3 54 $ 4 F4 R LL % 5 F5 T G B) Do your data support unidirectional or bidirectional replication of the viral genome? How have you come to this conclusion? A 6 MacBook Air F6 Y H & 7 ◄◄ F7 U * 00 8 J DII F8 - ( 9 K DD F9 0 ) 0 L A F10 P
Transcribed Image Text:Question 2. For your senior research, you end up studying the life cycle of an animal virus whose genome consists of a single circular, double-stranded DNA molecule. Your project is to define the number and location of the origin(s) of replication and to determine whether replication proceeds in one (unidirectional) or both (bidirectional) directions away from an origin. To accomplish this, you isolate many identical strands of DNA that have already been partially replicated. You cleave each piece of DNA exactly once with a restriction enzyme. You then observe the cut pieces of DNA using an electron microscope. Below is a schematic representation of what you observe. Remember that each line represents a different piece of DNA, and not a fragment of a larger piece. Using this data, answer the following questions: A) How many origins of replication do you think the viral genome has, and why do you think this? 30: F2 #3 *** E D 80 F3 54 $ 4 F4 R LL % 5 F5 T G B) Do your data support unidirectional or bidirectional replication of the viral genome? How have you come to this conclusion? A 6 MacBook Air F6 Y H & 7 ◄◄ F7 U * 00 8 J DII F8 - ( 9 K DD F9 0 ) 0 L A F10 P
Expert Solution
Step 1

Introduction

Replication is a process by which DNA strands synthesize a complementary strand of itself, to ensure that after cell division each new daughter strand has at least one of the parental strands.

Replication begins at a point called the origin of replication(ori). A genome can have single or multiple ori.

When replication begins at ori, a replication bubble or eye is formed. The replication proceeds from this replication bubble and continues until the whole strand is replicated.

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