Question 1 In the second step of lariat splicing the 2'OH group of a G in the branch point attacks the 3' splice site the 3' OH group of the first exon attacks the 3' splice site the 3' OH of the first exon attacks the 5' splice site none of the above

Question 6. How does the sequence of the primary transcript resemble the sequence of the gene encoding it?

Question 7. What are the first three amino acids in the protein that is produced from this gene? -35 sequence Pribnow box 5' GATTCCGTATTACAGCATAGGCTATATTCACGTGGATGGCTAGT... 3' 3' СТААGGCAТААTGTCGTATСCGATATAAGTGCACCTАCCGAТСА. .. 5' Start site

QUESTION 19 Which of the following is the start codon?     GUC     CCC     UAG     AUG

Question 4. Given the probability that a restriction enzyme will cut DNA if the recognition sequence for the enzyme is 5'-GGATCC-3' and the GC content is 60%, c) Which of the following will lead to DNA cutting more frequently? Choose all that apply. O Increasing the AT content to 60% O Using a seven base cutter with the sequence 5'-GGATCCC-3' O Using a five base cutter with the sequence 5'-GGACC-3' O Increasing the GC content to 70% O Decreasing the GC content to 50%

QUESTION 45 Gene edits can be made to Eukaryotic DNA by using a complex composed of: Progenitor cell and 4 master regulatory genes Cyclic AMP and CRP protein Ubiquitin protein and CRISPR Guide RNA and Cas 9 protein Histone proteins and methyl groups, QUESTION 46 Click Saye and

Question 1: The restriction enzyme Sau3A recognizes the sequence 5'-/GATC-3' and cleaves on the 5' side of the G as indicated by the slash. Since the top and bottom strands of this restriction site are identical in the 5'-3' direction, only one strand of the site needs to be shown. The single-stranded ends produced by Sau3A cleavage are identical to those produced by BamHI, which recognizes the sequence 5'-G/GATCC-3' and cuts at the location of the slash. This means that an end cut by one enzyme can be ligated onto an end cut by the other enzyme by DNA Ligase. Given this information, answer the following questions: A) Write out both strands of the recognition sequences for BamHI and Sau3A, show what they would look like after they are cut, and what the DNA would look like after you ligate a BamHI site to a Sau3A site. Make sure that it is easy to follow the individual pieces of DNA. You may want to use two different colors to keep them separate. B) What fraction of BamHI sites can be…

List and describe the phases of meiosis.