Purification of a new unknown protein that you isolated from tissue and Assume that you have reached the following data during the characterization; Gel filtration: Gel filtration in protein native conformation When chromatographed, it has a molecular weight of 240000 daltons (240 kDa) is detected to be around. Gel filtration: The same protein is first denatured with 6 M guanidinium hydrochloride subjected to gel filtration chromatography again under denatured conditions. is retained, and the only column from the column with a molecular weight of about 60000 daltons (60 kDa) a protein is obtained. SDS-PAGE: Protein finally SDS-PAGE in the presence of beta-mercaptoethanol (Sodium dodecyl-sulphate polyacrylamide gel electrophoresis) analysis being held. As a result of SDS-PAGE analysis, their weight in the gel is approximately 40000 daltons. Two protein bands corresponding to (40 kDa) and 20000 daltons (20 kDa) is observed. In the light of these findings, the quaternary/quaternary structure of the protein you isolated Please define. Explaining separately what you learned from each experimental finding Show how you came to the conclusion

Q: Given: Cryo-EM structure of PCoV_GX spike glycoprotein 1. What can you tell me about the identity…

A: Viruses are non-cellular particles that need a host cell to survive. Viruses are not considered…

Q: Subject: Biochemistry, chemistry, polyacrylamide gel electrophoresis Differentiate native PAGE and…

A: PAGE/SDS-PAGE are the electrophoretic techniques used for protein separation

Q: 1. Subunit Composition of a Protein A protein has a molecular mass of 400 kDa when measured by gel…

A: Gel electrophoresis is a laboratory method that can be used for sorting protein mixtures by…

Q: Red blood cells are normally circular in shape. Sickle cell anemia is a disorder that affects the…

A: sickle cell anemia is a genetic disorder caused by alternation in genetic sequence .

Q: Pick all that are TRUE regarding analysis of quaternary structures of proteins  using polyacrylamide…

A: SDS PAGE is Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis. The first statement is true…

Q: Determining the amino acid sequence in a protein usually in- volves treating the protein with…

A: Treatment of a polypeptide with eleven amino acids with one reagent gives the following fragments :…

Q: we need to get the three pka values of Arginine on this graph so we need the pka (COOH) pka( NH3+)…

A: Within the usual aqueous pH range, triprotic amino acids have an extra group as part of their…

Q: Protein purification table: A 50 ml crude skeletal muscle extract contains 32mg of protein per ml.…

A: Amount of initial extract formed = 0.14 µmol /minute. Protein = 32mg/ml,  given then for 10…

Q: Topic: Determination of Protein Concentration by Spectrophotometry: The Standard Curve   The use…

A: Beer lamberts law is the basis of spectrophotometry. The principle of spectroscopy is based on the…

Q: The purpose of SDS in an SDS-PAGE gel is to denature the proteins attach the proteins to the gel…

A: Proteins are the polymer of amino acids. They are joined by peptide bonds.

Q: The number of distinct protein folds in limited.  Why might this be so?

A: Protein folding is the physical process by which a polypeptide folds into its characteristic…

Q: Choosing the proper buffer solution In Protein Precipitation, two liters of 5mM buffer solution…

A: The processes that occur in the body require a specific and stable pH range. Buffers are solutions…

Q: Human hemoglobin as a tetrameric protein of about 64.5KDa consists of 2 beta chains. Calculate the…

A: Hemoglobin : It is a iron containing metalloprotein found in red blood cells . Hemoglobin in the…

Q: What are peptides? Do all proteins possess peptide bonds? Do all proteins respond to Biuret’s test?…

A: Since we only answer up to 3 sub-parts, we’ll answer the first 3. Please resubmit the question and…

Q: Protein: QQICIMFELTQISS Predict the products of the following reactions with the protein given, if…

A: Introduction The above protein can be decoded by using the one-letter code table. The given proteins…

Q: Loop regions play important roles in the secondary structure of protein. Define loop  region and…

A: Protein is a naturally occurring polymer that is made up of amino acids linked by peptide bonds.…

Q: Hydropathy plot analysis of your protein of interest reveals a single, prominent hydrophobic peak.…

A: The hydropathy plot, in the case of proteins, gives the idea about the hydrophilic and hydrophobic…

Q: High salt concentrations tend to cause protein aggregation. Suggest a way to identify proteins…

A: Salting out is a process based on protein solubility. The globular proteins are precipitate in the…

Q: Functional conformation: Ultimately the functional conformation of a protein is determind by its…

A: The conformation of protein can be defined as an organization of the overall molecular shape in the…

Q: CENTRAL DOGMA & STRUCTURE-FUNCTION RELATIONSHIPS IN PROTEINS You are a bacteriologist studying a…

A: In central dogma process, Information from DNA in the form of Nucleotide sequence it is transcribed…

Q: Simple problem-solving: Imagine you did a Bradford assay, and measured the absorbance of a set of…

A: Bradford assay is used to measure the protein concentration in the unknown samples. The standard…

Q: SDS-PAGE gels separate proteins by charge.  Therefore proteins that are 10,000 daltons (g/mol) will…

A: The Sodium Dodecyl Sulphate–Polyacrylamide Gel Electrophoresis (SDS-PAGE) is a discontinuous…

Q: A protein gives, under conditions of buffer composition, pH, and temperaturethat are close to…

A: SDS-PAGE is an electrophoretic technique that separates proteins based on their weight. BME is a…

Q: under certain conditions alpha helical bundles can form and can facilitate protein insertion into…

A: Integral membrane proteins fold into their active conformations in a complex milieu dictated by the…

Q: Low-resolution X-ray diffraction analysis of a protein composed of long stretches of the sequence…

A: The X-ray diffraction is one of the analytical techniques in which the X-rays are used for analysis…

Q: GABA (B) protein  Need help answering questions about the GABA(B) protein. What is the primary…

A: protein has 4 structures- primary secondary tertiary quaternary

Q: Some proteins migrate anomalously in SDS-PAGE gels. For instance, the molecular weight determined…

A: SDS-PAge is the method that separates the proteins on the basis of their molecular weight. It is an…

Q: DNA True or false A long chain polymer in which the internucleotide linkages are of the diester…

A: DNA is double-stranded in which two strands run in opposite directions. The secondary structure of…

Q: Describe what these experiments tell you about the structure of the proteins.

A: Gel filtration chromatography- Components are separated based on their difference in molecular…

Q: 9. Protein denaturation and renaturation. Probability of successful protein renaturation. Reversible…

A: Denaturation of protein occurs due the influence of denaturants on protein. Denaturants can be heat,…

Q: protein. How would the protein produced from sequence #1 differ from the protein produced from…

A: The sequences provided are of a non-coding strand or template strand. mRNA is transcribed…

Q: Polymerization of tubulin subunits into microtu-bules occurs with an increase in the orderliness of…

A: A cell is a mass of cytoplasm that is bound remotely by a cell layer. Generally minute in size,…

Q: 3. Approximate molecular weight for an unknown protein from gel-filtration experiment is 130 kDa.…

A: Given information : Molecular weight of the protein estimated by gel filtration chromatography = 130…

Q: purified Protein X' and you want to know its concentration. As we learned, you can calculate…

A: Absorbance = 0.6 Absorptivity = 0.2 ml/mg cm Path length = 1cm

Q: Why is picric acid and tannic acid used in treatment of burns?

A: Picric acids are organic compound, which has several important roles such as antiseptic drug,…

Q: Affinity purification: Assemble the following components to set up affinity chromatography to purify…

A: Affinity chromatography: It is a chromatographic technique for the separation of the compound on…

Q: Peptide mass determination. You have isolated a protein from the bacterium E. coli and seek to…

A: Given values: The expected mass of the peptide fragment = 739.38 The observed mass of the peptide…

Q: Create a ROUGH SKETCH (no need for exact hydropathy indices and residue numbers) of the hydropathy…

A: A hydropathy plot is an analytical tool that lets us quantitatively determine the hydrophobic or…

Q: The temperature of unfolding (denaturation) for a particular protein is 50 oC. A replacement of…

A: Thermodynamics of protein folding involves measurement of a protein where structural changes in a…

Q: I'm conducting an experiment concerned with the binding affinity of tretinoin (all trans retinoic…

A: Fluorescence resonance energy transfer (FRET) is a mechanism of energy transfer between two light…

Q: 1CFD CALCIUM-FREE CALMODULIN How long is the protein’s primary sequence? Does this protein have any…

A: Calcium-free (CaM) calmodulin is considered as the multifunctional protein which is also said as…

Q: the relationship between mutation of RECQ5 and human disease, in particular exploring the effects on…

A: RECQ5:- it is a type of helicases. It is an ATP dependent helicase that can binds with single…

Q: disadvantage of using protein blast

A: Proteins are one of the major molecules made up of several amino acids linked together by a peptide…

Q: Denaturation is basically the dis arrangement of intramolecular interactions in a tertiary structure…

A: 35. True,  Denaturation is a process in which proteins lose their native conformation i.e. normal,…

Q: Peptide Sequencing 1. A sample of an unknown peptide was divided into two aliquots. One aliquot w…

A: Cyanogen bromide (CNBr) cleaves at methionine (Met) residues mostly in a given polypeptide chain.…

Q: Not yet answered Protein X and Y are mixed together. Protein X is 400 residues in size, and…

A: Chromatography It is a laboratory technique through which a mixture is separated into its…

Q: The purification continues with a cation exchange step in which the positively charged cytochrome C…

A: A linear XY linear graph for the equation. A straight line "y = mx + b" where y = absorbance at 595…

Q: topic: Bradford Assay There are numerous methods of protein determination in use, but this module…

A: The Bradford assay is a dye-binding assay that is based on the changes in color of the Coomassie dye…

Proteins

We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.  

Protein Expression

The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.