Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
. Pick all that are TRUE regarding analysis of quaternary structures of proteins
using polyacrylamide electrophoresis:
I. The added β-mercaptoethanol disrupts S--S bonds bridging the
polypeptide chains causing the appearance of higher Rf bands
compared to the native protein run.
II. Heating up any protein before subjecting to SDS-PAGE will always
result in the formation of more than one band.
III. A good asymmetrical gel layout would be : (Lane 1) MW ladder, (2)
native protein, (3) protein + β-ME, (4) protein + HCL, (5) protein +
β-ME + HCl.
IV. Formation of a single band in the protein + β-ME + HCl run, whose
Rf is lower than the native run, could be indicative that the protein is
a homodimer.
A. I only
B. I and II
C. II and IIV
D. None is true
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