3. Approximate molecular weight for an unknown protein from gel-filtration experiment is 130 kDa. Thirty six mg of this pure protein was treated with excess of fluorodinitrobenzene. After the reaction and complete acid hydrolysis the mixture was found to contain 356 µg of dinitrobenzene derivative of methionine (free acid) and no other amino acid-dinitrobenzene derivatives. Is this protein consisting of single polypeptide chain or multiple subunits? If it consists of multiple subunits, then how many? From these data, can you calculate the molecular weight of the protein more precisely?

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Q: VII. Analysis of a peptide antibiotic purified from a strain of Bacillus brevii resulted in the…

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Q: If the absorbance at540nm of a biuret and protein solution containing 0.25mg/ml of protein .24 the…

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Q: 16. A quantitative study of the interaction of a protein with its ligand yielded the following…

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Q: Suppose you have two genetic variants of a large protein that differ only in that one contains a…

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Q: a protein pretreated with an sds page detergent shows two bands around 100 and 150 kD on the…

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Q: High salt concentrations tend to cause protein aggregation. Suggest a way to identify proteins…

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Q: 1. Consider the peptides P-A-I-G-E and M-A-T-T. a. Draw the structure of each peptide at a pH of 7.0…

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A: Mass spectrometry is a measured charge to mass ratio.

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Q: affinity

A:

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Q: Serum Albumin (Monomer) Ovalbumin 66,000 43,000 32mm 39mm 60mm 26mm 14,400 Lysozyme Unknown 1 ?…

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Osmosis

In simple words, osmosis is the slow, gradual movement of a liquid through a membrane. But there are nuances to this process. For instance, there has to be an imbalance in the distribution of solute in the liquid on either side of the membrane, which has to be semipermeable. The liquid in the region with a lower solute concentration moves to the region with a higher concentration, thereby displacing the solution in the process. The objective is to equalize the concentration of molecules on both sides of the membrane; the displacement of liquid can be viewed as a byproduct of this equalization. Since there is no energy required to carry osmosis, it is considered a passive process.