topic: Bradford Assay There are numerous methods of protein determination in use, but this module focuses on the Bradford assay. The Bradford assay is a dye-binding method that employs Coomassie Brilliant Blue G-250, whose structure is shown in Figure 2.3.4.1. Coomassie Brilliant Blue G-250 is a dye that interacts with proteins through hydrophobic and electrostatic interactions.     What are the identities and functions of the components of the Bradford reagent in protein content determination?

Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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topic: Bradford Assay
There are numerous methods of protein determination in use, but this module focuses on the Bradford assay.
The Bradford assay is a dye-binding method that employs Coomassie Brilliant Blue G-250, whose structure
is shown in Figure 2.3.4.1. Coomassie Brilliant Blue G-250 is a dye that interacts with proteins through
hydrophobic and electrostatic interactions.

 

 

What are the identities and functions of the components of the Bradford reagent in protein content
determination?

GI I
HO;S
-SO
HN-
Figure 2.3.4.1. Structure of Coomassie Brilliant Blue G-250.
Transcribed Image Text:GI I HO;S -SO HN- Figure 2.3.4.1. Structure of Coomassie Brilliant Blue G-250.
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