topic: Bradford Assay There are numerous methods of protein determination in use, but this module focuses on the Bradford assay. The Bradford assay is a dye-binding method that employs Coomassie Brilliant Blue G-250, whose structure is shown in Figure 2.3.4.1. Coomassie Brilliant Blue G-250 is a dye that interacts with proteins through hydrophobic and electrostatic interactions. What are the identities and functions of the components of the Bradford reagent in protein content determination?
topic: Bradford Assay There are numerous methods of protein determination in use, but this module focuses on the Bradford assay. The Bradford assay is a dye-binding method that employs Coomassie Brilliant Blue G-250, whose structure is shown in Figure 2.3.4.1. Coomassie Brilliant Blue G-250 is a dye that interacts with proteins through hydrophobic and electrostatic interactions. What are the identities and functions of the components of the Bradford reagent in protein content determination?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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topic: Bradford Assay
There are numerous methods of protein determination in use, but this module focuses on the Bradford assay.
The Bradford assay is a dye-binding method that employs Coomassie Brilliant Blue G-250, whose structure
is shown in Figure 2.3.4.1. Coomassie Brilliant Blue G-250 is a dye that interacts with proteins through
hydrophobic and electrostatic interactions.
What are the identities and functions of the components of the Bradford reagent in protein content
determination?
Transcribed Image Text:GI I
HO;S
-SO
HN-
Figure 2.3.4.1. Structure of Coomassie Brilliant Blue G-250.
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