Production of Bubbles Experiment Rate of Reaction (mm) Control #1 5 13mm 0 Omm Control #2 Plant vs Animal Catalase (Liver) 5 82mm Plant vs Animal Catalase (Potato) 0 10mm Concentration of Substrate/Enzyme (Used Liver) 5 83mm (Used H202) 50 2mm Temperature Boiling Water Bath 5 9mm Ice Water Bath pH 0 16mm HCI 5 45mm NaOH 5 79mm Water (pH 7) 0 1mm
using the method for experiment below and the table conduct 1 graph of the different factors vs rate of enzyme activity.
The experiment began by preparing a hot water bath by boiling water and an ice water bath using ice in a 400 mL beaker. In the control group, 2 mL of 3% H2O2 was placed in a test tube and a pinch of MnO2 was added. The rate of this reaction was assigned as 5, and the production of bubbles in millimeters (mm) was noted. The reaction was considered complete when no more bubbles were produced. Another control group was set up by placing 2 mL of 3% H2O2 in a test tube and adding a pinch of sand, with the
To investigate the difference between plant and animal catalase, 2 mL of H2O2 was added to a test tube and a small piece of fresh liver was added. The rate of reaction between 0-5 was noted, along with the production of bubbles in mm. The same procedure was repeated using a small piece of fresh potato.
Next, the effect of substrate/enzyme concentration was examined. 2 mL of H2O2 was placed in a test tube and a small piece of fresh liver was added. The reaction was allowed to complete, and then the liver was removed, keeping the H2O2 for the next step. 2 mL of fresh H2O2 was added to the test tube containing the used liver from the previous step, and the rate of reaction between 0-5 was noted. Additionally, a small piece of fresh liver was added to the used H2O2 in the test tube from step 6, and the rate of reaction between 0-5 was recorded.
For the temperature experiment, 2 mL of H2O2 was placed in a test tube and the test tube was briefly submerged in the boiling water bath to heat up. Then, a small piece of fresh liver was added, and the rate of reaction between 0-5 was observed. The same procedure was repeated using an ice water bath instead of the boiling water bath.
Lastly, the effect of pH was investigated. Three test tubes were prepared, each containing a sample of ground liver. To test tube #1, 1 mL of 0.1 M HCl was added, while test tube #2 received 1 mL of 0.1 M NaOH, and test tube #3 was filled with 1 mL of distilled water. pH paper was added to each test tube, followed by 2 mL of H2O2. The rate of reaction between 0-5 and the production of bubbles in mm were recorded for each test tube.
Step by step
Solved in 2 steps with 1 images
