Based your understanding of enzyme kinetics and inhibitors which line is the assay with the inhibitor? top line on the right side of the graph bottom line on the right side of the graph b) Determine the scale on the x and y axes and provide the coordinates for the point corresponding to 33 nM substrate on the without inhibitor curve. X: Y: c) What type of inhibition could be demonstrate by this graph? (select all that apply) competitive

4. Graphing the results from kinetics experiments with enzyme inhibitors The following kinetic data were obtained for an enzyme in the absence of inhibitor (1), and in the presence of two different inhibitors (2) and (3) at 5 mM concentration. Assume [Er] is the same in each experiment. [S] (mM) 1 2 4 8 12 1 2 4 8 12 [S] (mm) a. Determine Vmax and Km for the enzyme. b. Determine the type of inhibition and the Kıfor each inhibitor. 1/[S] M-1 Plot these data as double-reciprocal Lineweaver-Burk plots and use your graph to answers a. and b. 1000 500 250 125 83.3 Answer: The data may be analyzed using double-reciprocal variables. For each [S] and corresponding u, we will calculate 1/[S] and 1/v. sed to get mo Helmien (1) v (μmol/mL sec 12 20 29 35 40 1/v (1) v (1) μmol/mL se mL-sec/umol C 12 20 29 35 40 Please explain how to get K 1/v 20 × 104 10 × 104 -400 8.33x104 5.00x104 3.45x104 2.86x104 2.50x104 Condition No inhibitor 0 5 mM inhibitor (2) 5 mM inhibitor (3) Plots of 1/v vs. 1/[S]…

Please answer C and D.

From the following data (ESSAY DPPH & METHANOL), make a graph of the adjusted model Concentration (x-axis) vs. % inhibition (y-axis), provide the equation that is given and explain its behavior in a broad way. Do this activity step by step please