Which of the following statements regarding size exclusion chromatography is false?     During size exclusion chromatography, the largest compounds elute out first.     The elution order of fully excluded compounds follows an inverse diagonal relationship with respect to elution volume.     During size exclusion chromatography, the smallest compounds elute out at the end.     The elution order of partially included compounds follows an inverse diagonal relationship with respect to elution volume.

Using the table below can you please help me answer the following. Based on your results, what can you conclude about the effect of enzyme concentration on reaction rate? Explain

In a typical enzyme-catalyzed reaction, what are the relative concentrations of reactants and products as compared to the enzyme concentration?

A continuous enzyme reactor consists of a stirred tank of volume 0.01 m3 through which liquid is pumped at 0.02 m3⁄h. The enzyme is immobilised onto non-porous, spherical support particles, and the contents are stirred well to ensure that the composition is uniform and that the particles remain in suspension. A plastic mesh has been placed in front of the exit pipe to prevent the particles from leaving the vessel. The reactor is used to carried out a 98% conversion of a substrate entering at 0.1 kmol m3 ⁄ . Michaelis-Menten kinetics apply, with the following enzyme characteristics: Turnover number = 1 × 10^3 s KM = 5 × 10−3 kmol m3 ⁄ Enzyme RMM = 65,000 (a) If the enzyme is stable, calculate the mass of enzyme that needs to be immobilised. (b) If the enzyme is not stable and experiences exponential denaturation with a half-life of 10 h at the reaction temperature, determine the denaturation constant. (c) How would you expect denaturation to affect the reactor performance?