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1.Define Bioprospecting.
2. What properties in microorganisms were researchers at SINTEF and NTNU looking for?
3. What made them select Micrococcus luteus as a candidate organism?
4. Describe the steps involved in genetic engineering, prior to commercial production of the potential `UVAblue’ sunscreen.
5. What damage do long wave UV radiations induce in human cells?
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- What properties in microorganisms were researchers at SINTEF and NTNU looking for? What made them select Micrococcus luteus as a candidate organism? Describe the steps involved in genetic engineering, prior to commercial production of the potential `UVAblue’ sunscreen. What damage do long wave UV radiations induce in human cells?propose a hypothesis regarding the organism (escherichia, staphylococcus, pseudomonas, bacillus) that will demonstrate greater resistance to UV and why? if the hypothesis is supported, what specific experimental results will be observed? and what serves as the control treatment?10) List down 3 different test animals and give samples for which purposes they can be used in testing of a designed biomaterials? 11) What does FDA abbreviation stand for? 12) Order for the below given steps prior to FDA approval ? Controlled clinical trials In vitro testing (“in glass") In vivo testing w/animal models of disease In vivo testing w/healthy animals
- 1.What types of damage to human tissues can result from prolonged exposures to UV radiation? What protective measures can be taken to limit these types of damage, both during the experiment and in everyday life? 2.Which B. megaterium culture, logarithmic or stationary phase, would show the best survival following exposure to UV radiation?During the anthrax crisis of 2001, public health officials urged citizens not to take the antibiotic ciprofloxacin as a preventative measure. Public health officials were concerned that overuse of ciprofloxacin would lead to resistant varieties of anthrax and other bacterial pathogens. Ciprofloxacin is one of the few readily available antibiotics that can treat anthrax. 1)Do you think that public health officials should more directly regulate the availability of ciprofloxacin to ensure its proper use? 2)Why?You perform a Kirby-Bauer assay with two antibiotics. Antibiotic X has a zone of inhibition of 9 mm. Antibiotic Y has a zone of inhibition of 11 mm. Which antibiotic is better at killing this particular microorganism? Group of answer choices 1Antibiotic Y 2Antibiotic X and Y, which have identical antimicrobial activities 3Antibiotic X4 4It is impossible to tell from the information given
- 3) What is the effect of time on the bacteriocidal effects of UV? The effect of distance?1. Make a timeline of the development and advancement of Microtome. Include the significant inventors and people who contributed in developing the microtome. 2. Illustrate the different types of Microtome. a. Identify its uses b. Identify its different parts and the uses of its parts c. Differentiate how this particular type of microtome being usedYou are tasked with cultivating the following microorganisms:A recombinant strain of E. coliA wild strain of Streptomyces clavuligerus• Suggest a storage method for each microorganism and justify your choice. You have at your disposal a refrigerator and an ultrafreezer, in addition to materials and conditions for handling microorganisms.• Propose a culture medium for each (choose a complex medium for one organism and a synthetic medium for the other)As a complement of nutrients, they must be indicated on the following scale: +++ : above 5 g/L++ : from 1 to 5 g/L+ : below 1 g/L• Describe the procedure for preparing and sterilizing the components of the culture media for a volume of 5.0 L• Make a bench process selection scheme, up to the step of selecting a benchtop reactor 5 L.
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