Mass spectral measurements were made on 10 replicate low concentration Cd-EDTA samples: 181.5, 148.5, 158.5, 173.5, 142.5, 211.9, 163.5, 168.1, 157.5, and 175.1 counts. Ten measurements of a blank had a mean measurement of 42.1 counts. A sample containing 2.00 µM Cd-EDTA gave a measurement of 1827 counts. Estimate the signal and concentration detection limits for Cd-EDTA. signal detection limit: counts concentration detection limit: µM

The students conducted the assay for LDH activity using the two serum samples. Each cuvette (path length 1 cm) contained 3ml of a suitable assay buffer (including pyruvate as substrate). 20 microlitres of either serum was added to the cuvette and the absorbance values immediately recorded at the optimum wavelength for a period of 5 minutes (absorbance readings taken every 30 seconds).   Protein concentration of serum sample (mg/ml) Change in absorbance at optimum wavelength per minute Control serum (C) 8 -0.04 Diseased serum (D) 7.8 -0.6 1c. Using the molar absorption coefficient of NADH as 6220 M-1 cm-1, and by application of the Beer-Lambert law, estimate the enzyme activity in the two samples (C and D). Express activity as moles per second.  1d. Estimate the specific activity of the two samples (moles per second per microgram).

Analyte in an unknown gave a signal of 9.3 mV.  When 100 mL of 0.211 M standard was added to 100.0 mL of unknown, the signal increased to 16.7 mV.  Find the concentration of the original unknown solution.

Provide a structure of the fragment in the mass spec producing the peak at 139 m/z and a structure for the fragment that was lost.

You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) Absorbancy 595nm 0 0.155 1 0.287 2 0.420 3 0.523 4 0.651 5 0.756 6 0.878 7 0.918 8 0.954 9 10 0.982 0.990 After blanking against a bradford-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.566 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg/ml (up to 3 decimal places).