A spectrophotometer method for the quantitative analysis of Pb2+ in blood uses Cu2+ as an internal standard. A standard containing 1.75 ppb Pb2+ and 2.25 ppb Cu2+ yields a signal of 0.832 for Pb2+ and a signal of 0.351 for Cu2+. A sample of blood is spiked with the same concentration of Cu2+, giving a signal of 0.520 for Pb2+  and 0.289 for Cu2+. Determine the concentration of Pb2+ in the sample of blood.

Appl Of Ms Excel In Analytical Chemistry
2nd Edition
ISBN:9781285686691
Author:Crouch
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Chapter12: Spectrochemical Methods
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A spectrophotometer method for the quantitative analysis of Pb2+ in blood uses Cu2+ as an internal standard. A standard containing 1.75 ppb Pb2+ and 2.25 ppb Cu2+ yields a signal of 0.832 for Pb2+ and a signal of 0.351 for Cu2+. A sample of blood is spiked with the same concentration of Cu2+, giving a signal of 0.520 for Pb2+  and 0.289 for Cu2+. Determine the concentration of Pb2+ in the sample of blood.

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