You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) Absorbancy 595nm 0 0.225 1 0.310 2 0.420 3 0.510 4 0.610 5 0.720 6 0.810 7 0.915 8 0.950 9 0.980 10 0.990 After blanking against a bradford-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.523 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).

You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) Absorbancy 595nm 0 0.225 1 0.310 2 0.420 3 0.510 4 0.610 5 0.720 6 0.810 7 0.915 8 0.950 9 0.980 10 0.990 After blanking against a bradford-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.523 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter9: Atomic Absorption And Atomic Fluorescence Spectrometry

Section: Chapter Questions

Problem 9.22QAP: The chromium in an aqueous sample was determined by pipetting 10.0 ml. of the unknown into each of...

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In a Bradford assay, 11 µl of a protein isolate sample was diluted by adding 21 µl of water prior to the addition 2.0 mL Bradford reagent. Calculate the protein concentration (in mg/mL) of the original protein isolate sample whose absorbance reading at 595 nm is 0.304 after dilution. The protein standard solutions used for the calibration curve of the Bradford assay was constructed using bovine serum albumin (BSA) stock solution whose concentration is 200 µg/mL. The concentration and the corresponding absorbance reading at 595 nm of the protein standard solutions are summarized in the table below: Standard soln # 1 2 13 4 6 conc of BSA 12.00 24.00 36.00 48.00 60.00 (pg/mL) Absorbance at 595 nm 0.000 0.156 0.249 0.328 0.411 0.528 zNote: Final answer format must be x.xxx (three decimal places). Round off only in the final answer. Do not round off in the middle of the calculation.
You obtained the following raw data when setting up a Biuret standard curve: Absorbancy 540nm BSA (mg/ml) 0.158 1 0.210 2 0.260 3 0.305 4 0.360 0.410 0.455 7 0.510 8 0.530 9 0.550 10 0.554 After blanking against a biuret-dH20 sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.279 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).
You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml) Absorbancy 540nm 0 0.158 1 0.210 2 0.260 3 0.305 4 0.360 5 0.410 6 0.455 7 0.510 8 0.530 9 0.550 10 0.554 After blanking against a biuret-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.251 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).
6. Blue Blue dye stock solution 0.293 M Absorbance at 630 nm 0.00265 Calibration curve y = 0.0833x A solution is prepared by diluting 2.79 mL of the blue dye stock solution to 25.00 mL. The measured absorbance for the prepared solution is listed in the data table. (a) What is the theoretical molar concentration? [Blue]theoretical x 10 |M (b) What is the experimental molar concentration? [Blue]experimental x 10 M (c) What is the percent error? Percent error (blue) = %
3. An indicator is a dye whose spectrum changes with pH. Consider the following data for the absorption spectrum of an indicator (pK = 4) in its ionized and nonionized form. InH+ → In + H* Molar absorptivity, ɛ (M1.cm1) InH+ (nm) In 400 10,000 420 15,000 2,000 440 8,000 8,000 460 12,000 480 3,000 The absorbance of the indicator solution is increased in a 1-cm cell and found to be 400 420 440 460 480 (nm) A 0.250 0.425 0.400 0.300 0.075 a. Calculate the pH of the solution. [4.0] b. Calculate the absorbance of the solution at 440 nm and pH 6.37 for the same total indicator concentration. [0.400] c. If you want to measure the quantum yield of fluorescence of In and H* independently, what wavelength would you choose for exciting In H+? For exciting In? Why?
In a Bradford assay, 17 pl of a protein isolate sample was diluted by adding 24 pl of water prior to the addition 2.0 mL Bradford reagent, Calculate the protein concentration (in mg/ml) of the original protein isolate sample whose absorbance reading at 595 nm is 0,384 after dilution The protein standard solutions used for the calibration curve of the Bradford assay was constructed using bovine serum albumin (BSA) stock solution whose concentration is 200 ug/mL The concentration and the corresponding absorbance reading at 595 nm of the protein standard solutions are summarized in the table below: Standard soln # 3 6 conc of BSA Kug/mL) 12.00 24.00 36.00 48.00 60.00 Absorbance lat 595 nm 0.000 0.156 0.249 0.328 0.411 0.528 zNote: Final answer format must be x.xox (three decimal places) Round off only in the final answer. Do not round off in the middle of the calculation
The absorbance of an unknown dye solution is measured to be 1.06 by a spectrometer. The calibration plot of the spectrometer is provided below. What is the molar concentration of the unknown sample (in mole L)? Absorbance vs. Molarity 18 y3 67024x-0.0343 16 14 12 1 0.8 0.6 0.4 0.2 01 0.2 0.000005 00001 0000015 Concentration of solutions, mol/L 0.00002 0.000025 000003 0 163e-5 O 2.35e-6 0 163 0 235 Absorbance
5. A solution of certain dye has a calibration graph shown below when measured at 425 nm in 1.00cm cells. A sample of the dye with an unknown concentration has an absorbance of 0.733. Determine the molar absorptivity of the dye at 425 nm and the concentration of the dye in the unknown solution. Absorbance vs. Concentration 1.200 1.100 1.000 0.900 0.800 0.700 0.600 0.500 0.400 1.50E-05 2.50E-05 3.50E-05 4.50E-05 5.50E-05 6.50E-05 Dye Concentration (Mol/L)
1. Data & Results 4.0 mglmL Concentration of stock solution (include units): Table 1-Beer's Law Plot Data V Volume of standard solution dispensed (mL) M, Diluted concentration (include units) Sample Absorbance A 2.00 mL 0.084 4.00 mL 0.173 l6.00 mL 0.259 8.00 mL 0.340 E 10.00 mL 0.418 Equation of trendline from Beer's Law plot: whats the diluted concentration and equation of trendline from the beers law plot once created?
This graph shows an absorbance vs concentration curve for Co2*(aq) solution. Sample Standard Curve 1.20 1.00 800 .600 a 400 e 200 20 02 0 080 100 Co2. conc. (M) .020 040 OGO 120 140 160 • Slope - 6.414 Intercept -7.799991E 03 Con. Coef - 9791 Assuming that Beer's Law holds, A = Elc, and the cuvette was a standard 1-cm optical path, what is the molar absorptivity coefficient for the Co2* solution? a. 3.58 x 103 cm" o b.3.58 x 103 cm M-1 OC 7.80 x 103 cm1M-1 O d. 6,414 cm1M1
A student weighed out 0.150 g of protein powder and dissolved it in 100 mL of water (Solution 1). The student then diluted this solution by transferring 1 mL into a 25 mL flask and diluting with water (Solution 2). Finally, 1 mL of that solution was transferred to a test tube and combined with 4 mL Bradford reagent. The absorbance of the solution in the test tube was 0.144. Assuming that the best fit linear line of the standard curve was y=0.04144x+0.01521 (μgmL), calculate the percent protein by mass in the original protein powder.
13. 0 0 0 5 The following curve was made by absorption spectrophotometry (2 = 500 nm) of standard protein solutions using the Lowry method you will perform in lab Exercise #4. If an unknown protein solution was analyzed using the same system and an absorbance value of 0.125 was measured, what was the protein concentration of the unknown? 1 Absorbance at 500 nm 0.8 0.6 0.4 0.2 y = 1.492E-03x + 3.513E-02 0 100 A. 107 μg/mL B. 83.7 μg/mL C. 71.4 μg/mL D. 60.2 µg/mL 200 300 400 500 600 Protein concentration, ug/mL