Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
Related questions
Question
It is true ddNTPs.....
use during sequencing DMA
lack 3'hydroxide present in dNTPs
none of above
both of above
Expert Solution
Step 1
dNTP( Deoxyribonucleoside Triphosphate) are the building blocks of the DNA and they have the 3'hydroxide group attached on the pentose sugar ring structure and ddNTPs(Dideoxyribonucleoside Triphosphate) lack this feature.
Thus, ddNTPs acts as chain elongating inhibitors( no further chain elongation can occur )of DNA polymerase during the Sanger Sequencing Technique.
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