. Below is a plasmid map of pBR328, describe its screening procedure. Ncol Mscl EcoRI BspEI Pvull Cm Pvul pBR328 4907 bps Amp Clal Hindill Pstl EcoRV BamHI Tet ColE1 ori Sall

Analyzing Cloned Sequences A base change (A to T) is the mutational event that created the mutant sickle cell anemia allele of beta globin. This mutation destroys an MstII restriction site normally present in the beta globin gene. This difference between the normal allele and the mutant allele can be detected with Southern blotting. Using a labeled beta globin gene as a probe, what differences would you expect to see for a Southern blot of the normal beta globin gene and the mutant sickle cell gene?

Provide a brief summary of the Sanger sequencing method.

Why is so much effort being poured into genome mapping applications?

Match the method with the appropriate enzyme. _____ PCR a. Taq polymerase _____ cutting DNA b. DNA ligase _____ cDNA synthesis c. reverse transcriptase _____ DNA sequencing d. restriction enzyme _____ pasting DNA e. DNA polymerase (not Taq)

Why are antibiotic resistance markers such as ampR important components of bacterial plasmid cloning vectors? a. The plasmid must have resistance to accept DNA inserts. b. They allow the detection of plasmids that contain an inserted DNA fragment. c. They ensure the presence of the ori site. d. They ensure that the plasmid can be cut by a restriction enzyme. e. They allow identification of bacteria that have taken up a plasmid.

Cloning Genes Is a Multistep Process In cloning human DNA, why is it necessary to insert the DNA into a vector such as a bacterial plasmid?

Which of the following can be used to carry foreign DNA into host cells? Choose all correct answers. a. RNA b. viruses c. PCR d. plasmids e. lipid clusters f. blasts of pellets g. xenotransplantation h. nanoparticles

In selecting target cells to receive a transferred gene in gene therapy, what factors do you think would have to be taken into account?

How has proteomics been used in cancer detection and treatment?