2. a. Research DNA quantitation by UV absorption. Explain what A230, A260 and A280 values represent. What should be the ratios of A260/A230 and A260/A280 in a well-isolated and purified DNA sample? b. How would these ratios change if the isolated sample had low concentrations of DNA? c. Assume the sample was isolated with phenol-chloroform- isoamyl alcohol method. How would these ratios change if the sample had phenol contamination in the end?

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