Drag and drop the appropriate text in the gaps below.Firstly, the plasmid DNA in the E. coli is propagated throughThen the bacterial cells arepelleted by centrifugation at 10,000 RPM. The media supernatant isremoved and the bacterial cells areThe bacterial cells are thenlysed via|. Contaminatingmacromolecules such as protein and chromosomal DNA is removed byOvernight incubation of the bacterial culturewashed in cell resuspension bufferaddition of lysis bufferaddition of neutralisation bufferwashing of the spin columnseparation via agarose gel electrophoresis

Joshua Lederberg created the term "plasmid" in 1952. Plasmids are genetic elements which are…

Answered: Drag and drop the appropriate text in…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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In transformation, donor__________ in the growth mediumenters a recipient cell.
PART C (cont'd) Which of the following steps (on this page and the next page) would be necessary to determine the most effective antibiotic for a bacterial infection using the Disk Diffusion Assay? Select three necessary steps and three unnecessary or incorrect steps, and explain why it is or is not necessarv/correct. Collect a sample of bacteria from the infected patient. Collect samples of bacteria from the bathroom taps in the patient's room. Collect samples of bacteria from other patients who seem to have the same infection. Soak disks in many different types of antibiotic. Soak disks in the antibiotic you have the biggest supply ot. Label the disks with the type of antibiotic you soaked them in.
Pipetting 1. Explain why a micropipettor is an important instrument in biochemistry labs. 2. Describe the basic components (and function) of a micropipettor. Bacterial Techniques 1. Define the following. (a) Serial Dilution (b) Streak Plating (c) Spread Plating Transformation 1. Define bacterial transformation and explain why it is an important method in biochemistry labs. 2. Describe (figure, narrative) a plasmid and describe the basic components of a plasmid. 3. What role does CaCl2 play in bacterial transformation? 4. What role does heat shock play in bacterial transformation? Plasmid Isolation 1. Describe the roles the following play in plasmid purification. (a) Lysis Buffer (b) Neutralization Buffer (c) Elution Buffer
PART C (cont'd) Which of the following steps (on this page and the next page) would be necessary to determine the most effective antibiotic for a bacterial infection using the Disk Diffusion Assay? Select three necessary steps and three unnecessary or incorrect steps, and explain why it is or is not necessarv/correct. Collect a sample of bacteria from the infected patient. Collect samples of bacteria from the bathroom taps in the patient's room. Collect samples of bacteria from other patients who seem to have the same infection. Soak disks in many different types of antibiotic. Soak disks in the antibiotic you have the biggest supply ot. Label the disks with the type of antibiotic you soaked them in. • Leave the petri dishes uncovered while the bacteria grow. , cover the petri dishes while the bacteria grow. Treat the bacterial culture with a disinfectant before spreading on the agar plate Wear personal protective equipment (gloves, mask, lab coat) while performing the procedure Wear…
Copy and paste the link below and watch the video on Youtube https://www.youtube.com/watch?v=8RBs0Ghg_48 Answer the following Questions: 1. What are the chemicals and materials used in gel electrophoresis? 2. Draw a schematic diagram of a gel electrophoresis set-up 3. Describe the procedure in doing a gel electrophoresis experiment. Why is there a need for a leveling bubble/leveler? What is the use of the rubber dam? 4. What is the use of ethidium bromide and why must you wear gloves when you handle it? 5. What makes the DNA fragment move towards the positive plate? 6. What is the purpose of glycerol in the sample buffer? 7. What is the use of a DNA ladder? 8. What will happen when you increase the voltage of the set-up? 9. Can gel electrophoresis be used to separate amino acids? If so, how is it done?
In the bacterial transformation experiment, what is the primary purpose of using an ampicillin-containing medium? LB agar is __________.
Complete the following tasks. You discovered that a species of bacteria can break down StyrofoamT (polystyrene) products due to an enzyme it produces, polystyrenase. You wish to study the gene that codes for this enzyme. Task 1: DNA Extraction To begin work on the bacterium, you begin by extracting its genomic DNA (GDNA). What is the purpose of the following procedures? Answer briefly but completely. Using sodium dodecyl sulfate, a detergent Answer: а. b. Adding RNase A and Proteinase K during extraction Answer: c. Adding ethanol before recovering the DNA extract С. Answer: Task 2: Polymerase Chain Reaction After purifying the gDNA extract, you want to isolate and amplify the polystyrenase gene. You perform PCR using the appropriate gene-targeted primers. What is the purpose of the following PCR components? Answer briefly but completely. DNA polymerase isolated from Thermus aquaticus Answer: а. b. Deoxynucleotide triphosphates (DNTPS) Answer: С. Forward and reverse primers Answer: Task…
uses of 1D and 2D polyacrylamide gel electrophoresis techniques.
Looking at the model data i upload and given the question how could i analyse tboth experimental results (antibiotic selection and agarose gel). Including one clearly labelled image of the agarose gel and with an accompanying legend. And how could i interpret the findings of the results and draw a conclusion i.e. what is in each tube? Also is there any further experiments that I could perform to confirm the identity of the plasmid stocks?
Special Media for Isolating Bacteria OBJECTIVES Because multiple methods and multiple media exist, you must be able to match the correct procedure to the desired microbe. For example, if bacterium B is salt-tolerant, a high concentration (>5%) of salt could be added to the culture medium. Physical conditions can also be used to select for a bacterium. If bacteri- After completing this exercise, you should be able to: 1. Differentiate selective from differential media. 2. Provide an application for enrichment and selec- tive media. um B is heat-resistant, the specimen could be heated before isolation. Dyes such as phenol red, eosin, or methylene blue are sometimes ineluded in differential BACKGROUND media. Products of bacterial metabolism can react with One of the major limitations of dilution techniques used to isolate bacteria is that organisms present in limited amounts may be diluted out on plates filled with dominant bacteria. For example, if the culture to be isolated has 1…
Give advantages and disadvantages of using commercially available kits for plasmid extraction over conventional alkaline lysis technique.
(ACADEMIC) Time The solid matrix that is usually used to attach the target DNA in southern blotting technique is usually a O a. Polyacrylamide gel O b. Petri dish Oc. Cell culture plate O d. Membrane Oe. Micro-titer plate CLEAR MY CHOICE

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