Spin 1.5 ml of cells in a microcentrifuge at maximum speed (12,000 rpm) for pellet. Remove the supernatant completely with a Pasteur pipet or a plastic pipet The spins can be performed at C or at room temperature. Longer spins make it a to resuspend cells. Resuspend pellet in 100pl GTE solution and let sit 5 min at room temperature. A cells are completely resuspended. Add 200al NaOH/SDS solution, mix by tapping tube with finger, and place on ice min. Add 150ul potassium acetate solution and vortex at maximum speed for 2s t Place on ice for 5-15 min. Be sure mixing is complete. Spin 3 min at 12,000 rpm to pellet cell debris and chromosomal DNA

Please answer fast Dilution Problem. A culture of Staphylococcus is diluted as follows: (1) 20mL are added to 80mL of water. (2) 10uL from (1) are then added to 9.99mL of water. (3) A 10-2 dilution is made from tube # (2). (4) 100uL from (3) are plated for a pour plate and incubated.   Growth Problem.A culture with approximately 2x103 cells/mL were incubated. After 3 hours, the number of cells had increased to 3x105. a) How long was the generation time in minutes?b) How many generations have occurred

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After trypsinization of cells in the flask and cell counting, you determined a concentration of 10 million cells/mL. What volume of this cell suspension will you use to prepare a 20 mL cell suspension (200,000 cells/mL) for a 24-well plate? O a. 0.4 ul b.0.4 mL Oc. 40 mL O d.40 µL e. none of the above