Spin 1.5 ml of cells in a microcentrifuge at maximum speed (12,000 rpm) for pellet. Remove the supernatant completely with a Pasteur pipet or a plastic pipet The spins can be performed at C or at room temperature. Longer spins make it a to resuspend cells. Resuspend pellet in 100pl GTE solution and let sit 5 min at room temperature. A cells are completely resuspended. Add 200al NaOH/SDS solution, mix by tapping tube with finger, and place on ice min. Add 150ul potassium acetate solution and vortex at maximum speed for 2s t Place on ice for 5-15 min. Be sure mixing is complete. Spin 3 min at 12,000 rpm to pellet cell debris and chromosomal DNA
Spin 1.5 ml of cells in a microcentrifuge at maximum speed (12,000 rpm) for pellet. Remove the supernatant completely with a Pasteur pipet or a plastic pipet The spins can be performed at C or at room temperature. Longer spins make it a to resuspend cells. Resuspend pellet in 100pl GTE solution and let sit 5 min at room temperature. A cells are completely resuspended. Add 200al NaOH/SDS solution, mix by tapping tube with finger, and place on ice min. Add 150ul potassium acetate solution and vortex at maximum speed for 2s t Place on ice for 5-15 min. Be sure mixing is complete. Spin 3 min at 12,000 rpm to pellet cell debris and chromosomal DNA
Biology: The Unity and Diversity of Life (MindTap Course List)
14th Edition
ISBN:9781305073951
Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Publisher:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Chapter8: Dna Structure And Function
Section: Chapter Questions
Problem 2DAA: HersheyChase Experiments The graph shown in FIGURE 8.5 is reproduced from an original 1952...
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