Indicate the contrast statements of the following chromatographic techniques of separation. A. A separation of components mixture based on migration rates in a stationary phase by a gaseous or liquid mobile phase. B. The supported materials coated of flat plate or in fibres of paper stationary phase is known as planar chromatography. C. The rate of migration of molecules or analytes is depending on the relative solubility of the solute in the stationary phase and mobile phase. D. Involve an electronic transition of valence shell electron in free atoms to provide analytical information about sample composition.
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- In gravimetric analysis, a colloidal product is obtained when Select one: a. The nucleation process is major b. The relative super saturation (RSS) is small Oc. Particles size exceeds 0.02 mm d. The concentration of solute at anytime is very small e. Crystal growth is predominantDetermine the amount of glucose in the unknown sample by plotting a standard curve of Absorbance at 620 nm on Y-axis and μg of glucose on the x-axis.Calculate the effective quantity (g) of sodium chloride related to tonicity in 100ml of an intravenous fluid labeled "5% dextrose in 0.45% sodium chloride," and indicate whether the solution is isotonic, hypotonic or hypertonic. The answer is Hypertonic and 1.35g NaCl but I don't understand how to get the answer.
- A pharmacist prepares 80 mL of an isotonic solution of 1.1 %w/v Lidocaine HCl (E-value 0.2). To prepare the solution, he dissolves Lidocaine HCl in purified water to make an isotonic solution. Then he dilutes the Lidocaine HCl solution with 0.9%w/v sterile sodium chloride to complete the formulation. How many milliliters of purified water is needed to make the formulation by this method?A mixture of lipids containing phosphatidic acid, cholesterol, testosterone, phosphatidylserine, andphosphatidylethanolamine was applied to a hydrophobic interaction chromatography column. Thecolumn was washed with a high salt buffer, and the lipids were then eluted with decreasing saltconcentrations. In what order would the lipids be eluted from the column? Explain your answer.The simple form of |Hoff equation is: II = [B]RT In this equation the [B] is the molar concentration of solute. So: n m [B] = v MV = cg /MA Where c, the mass concentration of the solute is in the total volume of solution and M, is the molar mass of the solute. This equation can be replaced in the previous one to get: RT II = MA In this equation molar mass of given solute can be detemined from the slope of the II vs Cz plot. This equation applies only to solutions that are sufficiently dilute to behave as ideal-dilute solutions. In the case of non-ideal solutions, however, the extended formula is: II = [B]RT{1+ k. [B] + n. [B]² + ...} Biological macromolecules dissolve to produce solutions that are far from ideal, but we can still calculate the osmotic pressure by assuming that the van't Hoff equation is only the first term of a lengthier expression: II [B]RT(1+ b. [B]) II = RT + bRT. [B] [B] II = RT + bRT./M. */Ma п RT ÞRT Ca MA MA In this equation molar mass of given biomolecule can…
- A mixture of lipids containing phosphatidic acid, cholesterol, testosterone, and phosphatidylcholine was applied to a hydrophobic interaction chromatography column. The column was washed with a high salt buffer and the lipids were eluted with decreasing salt concentrations. In what order would the lipids be eluted from the column? Explain your answer.Which of the following options shows the correct order of fastest to slowest motion of chloride ions, glycine and proteins through the separating gel? Chloride ions > Proteins > Glycine Glycine > Chloride ions > Proteins Chloride ions > Glycine > Protein Protein > Chloride ions > Glycine You are trying to separate a mixture of AMP, ADP, and ATP using ion exchange chromatography. Select appropriate supplies for your experiment. A positively charged column, deprotonated buffer, NaCl of same concentration. A positively charged column, protonated buffer, NaCl of increasing concentration. A negatively charged column, protonated buffer, NaCl of increasing concentration You cannot separate nucleotides using ion exchange chromatography.Drug X is intended to be formulated as tablets of 250 and 500 mg doses. The lowest value of aqueous solubility of the drug is 25 mg % over the pH range 1-8 and the permeability is 75%. According to BCS, this drug is classified as: * 8
- The reults for the macroscopic part: 0.30M glycerin – solution was translucent (could see text behind the test tube) 0.15M NaCl – solution was opaque (could not see text behind the test tube) 0.30M NaCl – solution was opaque (could not see text behind the test tube) 0.15M glucose – solution was translucent (could see text behind the test tube) 0.30M glucose – solution was opaque (could not see text behind the test tube) 0.30M Urea – solution was translucent (could see text behind the test tube) Results for microscopic part: 0.30M glycerin – no cells present 0.15M NaCl – normal sized cells 0.30M NaCl – crenated (shrunken and star-shaped) cells 0.15M glucose – no cells present 0.30M glucose – normal sized cells 0.30M Urea – no cells present Determine the osmolarity (hypoosmotic, isosmotic, or hyperosmotic) and tonicity (hypotonic, isotonic, hypertonic) of the following solutions.In which solutions did the osmolarity NOT match the tonicity? For those solutions, why did the osmolarity…The Cavity Ring-Down Spectroscopy (CRDS) technique offers greater sensitivity compared to other conventional spectroscopy techniques. Explain the underlying mechanism of this.1- Are the following changes in conditions likely to increase or decrease retention time? a. faster flow rate b. longer column c. longer connector between column and detector d. higher analyte concentration e. higher temperature f. larger sample size g. using a less polar column while doing a reverse-phase separation h. using a less polar mobile phase while doing a reverse-phase separation 2- In what order will the following substances elute? • In reverse-phase liquid chromatography: a. butylamine, 2-butylene, ethylamine, ethylene b. benzene, chlorophenol, phenol, trichlorophenol • In gas chromatography with a polar column: c. butanol, methanol, water • In gas chromatography with a nonpolar column: d. butanol, methanol, water • In ion chromatography: e. bromide, fluoride, sulfide