Q: What is the BEST glassware that could be used to hold the agar powder while weighing? After 24…
A: As per our guidelines, we are allowed to answer only one question. Kindly upload the rest of the…
Q: What is the function of the mordant used in the Gram staining procedure? If the iodine step is…
A: Gram stain or Gram staining, likewise called Gram's strategy, is a technique for staining used to…
Q: Explain why only gram-negative cells undergo decolorization during the gram staining procedure.
A: Gram staining is a method in which cells stain either pink (gram-negative) or purple (gram-positive)…
Q: The gelatin medium required inoculation with 3 loopfuls of culture. Why was so much organism…
A: Gelatin hydrolysis test is carried for detecting the ability of the bacteria to produce protease…
Q: Why don't you get isolated colonies from the pour plate if the broth culture was not diluted first?
A: The pour plate method is a technique used to count the number of colony-forming bacteria found in a…
Q: The acid fast bacterium, Mycobacterium smegmatis, is relatively safe for students to work with…
A: Mycobacteria are immobile, slow-growing (doubling time = 24 h), rod-shaped, gram-positive bacteria…
Q: In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside…
A: Several laboratory techniques necessitate smear preparation. A smear can be made using either broth…
Q: Give two reasons for heating the slide after the smear is air dried?
A: Smear is the thin film of the sample that is spread over the slide to be observed in the microscope.…
Q: Before inoculating and pouring molten nutrient agar into a plate, why must the agar first be cooled…
A: Agar or agar-agar is a jelly-like substances obtained from red algae. Agar is a solidifying agent…
Q: In Gram staining technique, Decolorizing agent step should take Select one: O a. 60 seconds O b. 30…
A: Usually, Acetone is used as a decolorizer but some prefer ethanol and acetone in a 1:1 ratio.…
Q: A powdered complex medium can be stored for months in the lab. However, after rehydrating the medium…
A:
Q: Bacillus subtilis (Gram-positive) and Pseudomonas aeroginosa (Gram-negative) bacteria are heat-fixed…
A: Bacteria have cell walls made up of polysaccharides that give them strength.Bacterial cell walls…
Q: What are pure cultures and why are they important? How are spread plates, streak plates, and pour…
A: In science and related fields, a culture plate is a low level lined lab holder for growing a layer…
Q: What are the advantages and disadvantages of the filter method in the analysis of water samples?
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Q: In centrifugation, the components of a given mixture are subjected to CENTRIFUGAL force, which…
A: Cell organelles include mitochondria, nuclei, vacuoles, ribosomes, plastids, peroxisomes, and…
Q: why it is useful to plot the microbial growth from a growing microbial culture?
A: The term microbial growth refers to the growth of a population of microbe, it is an increase in the…
Q: microbiology: Aseptic Culture Techniques Lab Explain why a loop or needle is flamed before it is…
A: The process of introducing a microorganism in a plate or a tube containing culture media is called…
Q: Can culture media be placed in a freezer? Why or why not
A: Culture media are also called as growth media. They are mixture of nutrients and other substances…
Q: You aseptically transfer 1 ml of your optional liquid culture into 99 ml of sterile water. What is…
A: The following are needed for calculating the dilution factor 1) Original volume of solution we…
Q: If you transfer 0.1 mL of culture into a 99mL of sterile water, then add 1mL of that to an agar…
A: Making a solution thinner or less dense is the procedure of dilution. Serial dilutions (also known…
Q: Why is it important to use a sterilized loop between streaks when preparinga streakplate?
A: Streaking is a technique used to isolate a pure strain from a single species of microorganism, often…
Q: When the number of colony-forming units per milliliter is calculated, you will focus your counts on…
A: Petri dish or cell culture dish is used to grow microbial cultures such as bacteria and fungi. The…
Q: Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?
A: In microscopic studies, the specimen needs to be prepared for an optimized vision under a…
Q: During the preparation of the capsule stain, the slide used to spread the India ink/bacteria mixture…
A: Capsule stain is a type of differential stain which uses acidic and basic dyes to stain the…
Q: When doing a gram stain in microbiology, one step can be eliminated and still allow distinction…
A: Please follow step 2 for detailed explanation.
Q: After performing the gram stain you observe your result as gram negative. To help confirm this…
A: Gram staining is a technique used for staining the microbial samples. It was performed by the…
Q: How is the correct way of incubating culture plate media? Explain why is that so.
A: After the way of life medium is set, and streaked with the necessary microbe/stock, the cover is put…
Q: You have a broth medium that you would like to turn into a solid medium in order to do streak…
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Q: chemically defined media b. complex culture media
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Q: You have a culture of cells at 3.5 x 108 cells/ml and you want to prepare a fresh 5 ml culture with…
A: The concentrated stocks can be diluted using the dilution formula. The dilution formula is:- C1V1 =…
Q: Put the following steps for running a gel in order. first Remove comb and put gel int v Choose ]…
A: Agarose gel electrophoresis is the technique that involves the separation of substances by providing…
Q: why In spread-plate technique, the bacterial suspension volume should not exceed 0.1ml.
A: Spread plate method is a technique which uses a Petri plate or dish in which a growth medium or gar…
Q: With liquid media such as broth, is it possible to determine if the culture is pure using the naked…
A: A culture is a method by which microbes are allowed to reproduce and multiply in predetermined…
Q: The culture you are working has a doubling time of 2 hours and a cell density of 3 x 106 cells per…
A: A serial dilution is a process of sequential dilution in order to decrease the density of cell…
Q: After performing the gram stain you observe your result as gram negative. To help confirm this…
A: Gram staining is a technique to identify bacterial stain gram-positive or gram-negative. It can be…
Q: In Gram staining method, list the reagents and state their role for each step of the procedure? In…
A: Bacteria are unicellular, prokaryotic organicism which are devoid of membrane bound organelles and a…
Q: What is the best streaking technique in a culture media? Explain
A: In microbiology, there are five I's that symbolise five different procedures used in the laboratory…
Q: You aseptically transfer 1ml of your original liquid culture into 999 ml of sterile water. What is…
A: The dilution factor may be expressed as the ratio of the concentration of stock solution to the…
Q: Why should your solvent be evaporated from the sample placed on the sterile disk before you place it…
A: The antimicrobial activity of natural compounds like essential oils derived from plants can tested…
Q: Describe the procedure for performing a Gram stain. What is the procedure? How does each component…
A: * Gram staining is used to test that whether f bacteria is positive or negative and also be used to…
Q: Why sometimes specimen may show organisms under the microscope but not appear in the culture media?
A: Introduction We are surrounded by various pathogens such as bacteria, viruses, fungus etc. Bacteria…
Q: You aseptically transfer 1 mL of your original liquid culture into 99 mL of sterile water. You then…
A: A serial dilution is any dilution in which the concentration is decreased by the same factor in each…
Q: Considering you can’t identify bacteria from a Gram stain, why might a physician perform a Gram…
A: Gram stain is a useful stain for identifying and classifying bacteria. It is a differential stain…
Q: Growth medium: You are running different experiments on microbes and need to make sure you choose…
A: A growth medium is a solid/ liquid media designed to support the growth of a population of…
Q: Why are plates incubated in a candle jar or CO2 incubator?
A: Plates contain the minimal media for growth of micro organism. The candle is burnt by utilising…
Q: Why should agar media be completely dissolved before they are dispensed in tubes and plates? What…
A: Introduction A growth medium, also known as a culture medium, is a solid, liquid, or semi-solid…
Q: To dilute a bacterial culture, 500 μl of a 16 hour culture is mixed with fresh culture media to a…
A: Given Values: Volume of the 16 hour culture = 500 μl Final Volume of the culture media = 5 ml
Q: Dilute cells from an old culture 1:50 into 200 mL cultures. What volume of the old culture would you…
A: Culture consists of appropriate substances which provides the growth to the desired organisms.
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- Why after a certain period the growth rate of the culture decreases until growth stops?What is the meaning of the word sterile? What would happen if culture mediafreshly prepared were not sterilized? Why should you always waitthe autoclave to drain all the steam (pressure gauge zero position) beforeto open the cover?Examine figure a, b (shown here). If you performed the quadrantstreak plate method using a broth culture that had 10× fewer cellsthan the broth used in the culture shown, which quadrant might youexpect to yield isolated colonies? Explain your answer.
- * types of condensers are Abbe O universial O achromatic all OOn agar plate does each discrete colony represent the growth of one cell? Explain your answer. Why can a single colony on a plate be used to start a pure culture?A PhD student leaving for vacation has asked an undergraduate student to perform daily media changes forhis iPSCs while he is gone. The culture is happening in 12-well plates where a volume of 2 mL is optimal. OnSaturday, when changing the media, the undergraduate decides to add 4 ml of media to the dishes (insteadof 2 ml) because he wants to skip lab and watch the Super Bowl on Sunday. He decides to add twice thevolume of media (4 mL) to tide the cells over till Monday. However, when the graduate student returns onMonday, he finds that some of his cells have died. Your job is to determine whether the cells died due to alack of oxygen. For the calculations that follow, diffusion and reaction occurs only in one direction. Also,assume that reaction only occurs at the cell-media interface. Use Michaelis-Menten type kinetics for oxygenuptake. You may use the following information:Ps = 150 mmHg (ambient oxygen tension)K = 1.19 nmol / mL / mmHg (solubility of oxygen in medium)D = 2…
- A PhD student leaving for vacation has asked an undergraduate student to perform daily media changes forhis iPSCs while he is gone. The culture is happening in 12-well plates where a volume of 2 mL is optimal. OnSaturday, when changing the media, the undergraduate decides to add 4 ml of media to the dishes (insteadof 2 ml) because he wants to skip lab and watch the Super Bowl on Sunday. He decides to add twice thevolume of media (4 mL) to tide the cells over till Monday. However, when the graduate student returns onMonday, he finds that some of his cells have died. Your job is to determine whether the cells died due to alack of oxygen. For the calculations that follow, diffusion and reaction occurs only in one direction. Also,assume that reaction only occurs at the cell-media interface. Use Michaelis-Menten type kinetics for oxygenuptake. You may use the following information:Ps = 150 mmHg (ambient oxygen tension)K = 1.19 nmol / mL / mmHg (solubility of oxygen in medium)D = 2…A PhD student leaving for vacation has asked an undergraduate student to perform daily media changes forhis iPSCs while he is gone. The culture is happening in 12-well plates where a volume of 2 mL is optimal. OnSaturday, when changing the media, the undergraduate decides to add 4 ml of media to the dishes (insteadof 2 ml) because he wants to skip lab and watch the Super Bowl on Sunday. He decides to add twice thevolume of media (4 mL) to tide the cells over till Monday. However, when the graduate student returns onMonday, he finds that some of his cells have died. Your job is to determine whether the cells died due to alack of oxygen. For the calculations that follow, diffusion and reaction occurs only in one direction. Also,assume that reaction only occurs at the cell-media interface. Use Michaelis-Menten type kinetics for oxygenuptake. You may use the following information:Ps = 150 mmHg (ambient oxygen tension)K = 1.19 nmol / mL / mmHg (solubility of oxygen in medium)D = 2…Why sometimes specimen may show organisms under the microscope but not appear in the culture media?
- If one is trying to determine if a culture is pure or mixed, which medium would you choose? Why? (best answer) agar plate; can compare colony morphology of isolated colonies O agar deep; can determine if pure or mixed based on presence or absence of bubbles and cracks O broth tube; can determine pure or mix based on presence or absence of pellicle, turbidity and sedimentWhy should bacterial culture plates be incubated inverted (bottom up)?during inoculation, the bacterial culture tube is always held at an angle and the lid of the Petri dish is slightly open. Explain the purpose of these steps briefly.