The acid fast bacterium, Mycobacterium smegmatis, is relatively safe for students to work with because of its thin cell wall. If a gram stain is to be performed on this particular microbe, it often takes the gram stain reaction. Why?

Q: Peptidoglycan on bacterial cell walls is stained by crystal violet dye and for this reason those…

A: True

Q: The number of bacteria in a sample can also be quantified with other techniques, such as examination…

A: In microbiology laboratories the number of microorganisms present in a particular sample is…

Q: what color would you expect for a gram positive microorganism for each incorrect mistakes while…

A: "Gram staining" is a technique used in microbiology. It is a method in which a bacterium can be…

Q: A microbiologist is working with a pure strain of bacteria isolated from a mixed sample. Which of…

A: Streptococcus mutans is a bacteria which is often found in the oral cavity of human. It is a…

Q: If you have a mixed (Gram positive and Gram negative) sample, and you complete a Gram stain, and all…

A: On the basis of lipid content in cell wall of bacteria these are classified into two types : Gram…

Q: A patient is diagnosed with a urinary tract infection and a urine sample is obtained and sent to the…

A: Question : A patient is diagnosed with a urinary tract infection and a urine sample is obtained and…

Q: Imagine a microbiologist performed a series of Gram stains but forgot to perform the destain step…

A: Bacteria are prokaryotic organisms that contain cell walls. Bacteria are further divided into…

Q: A colleague performs a smear prep from a colony on an agar plate. Without staining it, he looks at…

A: Gram staining is a common laboratory technique in microbiology that is used to differentiate…

Q: An acid-fast staining method was performed on a bacterial smear using the dyes carbolfuchsin and…

A: Introduction: Acid-fast staining is a type of differential staining technique that was first…

Q: Please discuss why staining is important in visualizing microbes under the microscope. What…

A: Staining is an essential step in visualising microbes under the microscope. THE NECESSITY OF…

Q: The KD of a given objective lens is 1.5 μm/ocular unit, a microbe spans 3 ocular units. What is the…

A: An objective lens is a lens system that is typically found on microscopes and other optical devices,…

Q: In the laboratory, autoclaving is required to prepare media for the growth of What type of method of…

A: The most effective method by which we sterilize the laboratory instruments is known as autoclaving.

Q: All of the following are correctly matched and can be found in bacteria EXCEPT: None of the other…

A: Note : Dear student as per our guidelines we are supposed to answer only one of your questions. So…

Q: Move the images of bacterial cells into the chart to illustrate the color of gram-positive and…

A: Gram staining is a technique used to differentiate between two groups of bacteria namely…

Q: In your own words, explain how gram staining is done and how it works.

A: Gram staining is a popular method for distinguishing between two large groups of bacteria based on…

Q: Suppose you are to observe an unknown bacterial slide. In your initial observation, it appears to be…

A: Bacteria are almost everywhere, but even with a microscope, they aren’t always easy to see. It is…

Q: If you Gram stain a bacterium that is gram-negative, which dye will be visible once the stain is…

A: Option b

Q: 45-year-old male had been working in his garden when he scratched the knuckle of his right arm…

A: There are many types of/species of bacteria. Through different techniques and lab procedures,…

Q: Why do you suppose endospore-forming bacteria are commonly found in the soil? Suppose your unknown…

A: Endospores are dormant, tough, vegetative structure produced by the bacteria from the Firmicute…

Q: Why is it sometimes difficult to attain both good resolution and good contrast at the same time for…

A: Magnification and resolution are important because the magnification in larges the image and the…

Q: Describe the shape and color of the following species of bacteria after Acid-Fast staining: E. coli…

A: Acid fast staining is a type of laboratory test that is used to determine if a sample of blood or…

Q: Supposed you are to observe an unknown bacterial slide. In your initial observation, it appears to…

A: Microorganisms are defined as organisms that cannot be viewed by unaided eyes and require a…

Q: Choose the dyes or reagents used in the Endospore stain. (More than one choices are correct)   a…

A: Simple stains- A stain that employs a single stain to color a bacteria: they are quick and easy.…

Q: The bacterial cells in the image below were stained with a high concentration of carbolfuschin for…

A: Carbol fuchsin, carbolfuchsin, or carbol fuchsin is basically a mixture of "phenol" and basic…

Q: one or your lab partners has followed the rrecommended procedure of running Gram-positive and Gram…

A: Gram staining is a common laboratory test to characterize the bacteria into gram-positive and…

Q: Explain the importance of rinsing with water every after each step in gram staining.

A: Introduction : Bacteria can be categorised in a number of ways depending on their morphology,…

Q: What color will a gram-negative cell be after Gram staining bythe conventional method?

A: Danish bacteriologist Hans Christian Gram developed the Gram staining method. This staining…

Q: Is is best to observe slides of bacteria stained with the acid fast stain under high dry…

A: The acid-fast stain is used to stain the acid-fast organisms. Acid-fast microorganisms have a thick…

Q: How is the correct way of incubating culture plate media? Explain why is that so.

A: After the way of life medium is set, and streaked with the necessary microbe/stock, the cover is put…

Q: The counterstain in the Gram stain is: A basic dye A negative stain A mordant An acid dye Necessary…

A: Staining is a method for enhancing contrast in material, usually on a tiny scale. Histology, as well…

Q: In a Gram-stained smear, medium-sized rods with rounded, pink ends were found, arranged chaotically.…

A: The Gram stain is a widely used method for differentiating between different types of bacteria. The…

Q: two different

A: Gram staining- A test that checks for bacteria at the site of a suspected infection such as the…

Q: Additional Differential Staining Procedures: Negative stain, Capsule Stain, Endospore stain,…

A: Staining is performed to enhance the sample present on a slide against its backdrop in such a way…

Q: A bacterial sample collected from a patient is processed using a Gram's stain. After staining, the…

A: Gram stain is a test that looks for bacteria in areas where an infection is suspected, such as the…

Q: Based from the results obtained from Gram staining, explain why bacterial cells retained or changed…

A: Staining is a technique that is used to enhance the contrast of the microscopic image through the…

Q: Picture that has dark background with red cells is the Negative stain and other picture in white is…

A: The Acid-Fast Stain is a lab test mainly used to find certain types of bacteria, like Mycobacterium…

Q: Materials: .Bacterial cultures: E. coli & Staphylococcus aureus • Crystal violet (primary stain)…

A: Introduction:- Staining is the biochemical technique for coloring the specimens. There are different…

Q: A throat and nasal sample are taken from a patient to determine the type of bacterial infection they…

A: Sputum Gram Stain test is a simple laboratory test which allows a doctor to diagnose any kind of…

Q: In the Gram stain, gram-negative bacteria appear red for all the foll

A: Gram-negative bacteria appear red because they do not retain the crystal violet stain used in the…

Q: Explain the different results in the Gram stain are due to differences in the structure of the cell…

A: There are many types of bacteria and bacterial species. They are prokaryotic cell which has a cell…

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.