The gelatin medium required inoculation with 3 loopfuls of culture. Why was so much organism required?

If you counted 300 colonies on a plate that had a 10-5 dilution of bacteria culture, how many colonies might you expect on a plate that had a 10-6 dilution of the same bacterial culture?

In performing the Kirby-Bauer procedure in a clinical laboratory setting, why must the agar be a certain depth? Why must the absorbance of the inoculum be standardized?

Why should bacterial culture plates be incubated inverted (bottom up)?

why In spread-plate technique, the bacterial suspension volume  should not exceed 0.1ml.

You plated 0.1 ml of a sample of kombucha diluted by a factor of 10^-3. It produced 43 colonies. What was the original concentration in the sample? Show your calculations.

The stool samples submitted by the two friends were enumerated following enrichment. The technician prepared serial dilutions. A volume of 1 mL of stool was mixed with 1 mL of diluent. From this dilution, they then took 1 mL and added it into 1 mL of diluent. The operation was repeated 5 more times. After finishing the serial dilution, 100 μ�L has been spread on different TSA plates. Following 24h incubation, the technician counted 23 colonies at the dilution 4.   What is the bacterial concentration in the enriched culture? [1 mark]

You have 2 mL of bacterial culture. How would you make a 10^-2 dilution using the entire culture volume

how to find out the test is differential or selective in the microbiology lab test?

Can you help me identify A and B?