In the determination of lead content in a solid food sample, 1 g of the solid food sample was extracted with 10 mL of solvent and then diluted to a final volume of 50 mL. A calibration plot of lead standards was obtained from the standard solutions of 1 to 50 ug/mL and it gave a linear regression line of y = 0.025x+0.004. The wavelength for lead analysis is 283.3 nm. The absorbance value for the extract was 0.302 arbitrary units. Calculate the percentage of Pb in 1 g of the solid food sample.

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In the determination of lead content in a solid food sample, 1 g of the solid food
sample was extracted with 10 mL of solvent and then diluted to a final volume of 50
mL. A calibration plot of lead standards was obtained from the standard solutions of 1
to 50 ug/mL and it gave a linear regression line of y = 0.025x + 0.004. The
wavelength for lead analysis is 283.3 nm. The absorbance value for the extract was
0.302 arbitrary units. Calculate the percentage of Pb in 1 g of the solid food sample.
Transcribed Image Text:In the determination of lead content in a solid food sample, 1 g of the solid food sample was extracted with 10 mL of solvent and then diluted to a final volume of 50 mL. A calibration plot of lead standards was obtained from the standard solutions of 1 to 50 ug/mL and it gave a linear regression line of y = 0.025x + 0.004. The wavelength for lead analysis is 283.3 nm. The absorbance value for the extract was 0.302 arbitrary units. Calculate the percentage of Pb in 1 g of the solid food sample.
Expert Solution
Step 1

The linear regression equation obtained from the calibration plot of the standard solution is:

y = 0.025*x + 0.004 

While plotting the graph, absorbance is taken in Y-Axis,  and lead concentration (μg/mL) is taken on X-axis. 

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