In gene cloning, a vector is required to transform the gene of interest into host cell. State the THREE (3) important regions of the plasmid. Elaborate your answer.
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
In gene cloning, a vector is required to transform the gene of interest into the host cell. The important regions of the plasmids include-
1. Origin of replication
Origin of replication, also known as Ori, is the site where the plasmid replication originated. This region contains specific DNA sequences. They are cis-acting sequences that help in the autonomous replication of the plasmid. During DNA replication, the DNA is unwinded at the Ori and Y-shaped replication forks are formed on both sides. It is important for the cloned plasmid to replicate to get a better yield during plasmid DNA extraction.
Step by step
Solved in 2 steps
