You want to clone the gene Dik1 into a plasmid that will be used to generate a transgenic mouse. The goal is for DIk1 to be expressed exclusively in the muscle. "MCK prom" denotes a promoter from a gene expressed exclusively in muscle. KanR is Kanamycin resistance gene, which acts as a selectable marker in this plasmid. Based on the illustrations of the plasmid and the Dlk1 gene, which restriction enzyme(s) should you use to clone Dlk1 into this plasmid? Konl BamHI Sall Psti EcoRI Kpnl ATGACT TACTGA. .TACTGA ATGACT 5° Kpni Bam ORI MCK prom -EcoRI Diki ORF 1.5 kb 重一 BamHI, Pstl Sall, Pstl Kpnl, Pstl Kpnl BamHI, EcoRI

Q: In the following "gene library" cloning experiment. Digested genomic DNA AmpR gene TCR gene TCR is…

A: Please follow step 2 for detailed explanation.

Q: recombinant plasmid

A: DNA is the material that carries all the information about how a living thing will look and…

Q: A plasmid is cleaved by restriction endonucleases and analyzed by agarose gel electrophoresis.…

A: From the agarose gel image, we get information about the plasmid size and the restriction sites…

Q: The table below shows restriction enzyme pairs used by various teams to digest the YFG and clone it…

A:

Q: nion and burger lover, your friend would like to incorporate the cheems gene (“cheM”) from the rare…

A: Transformation is a process in which the original DNA is transformed by the addition of foreign DNA…

Q: A plasmid that is both ampicillin and tetracyclineresistant is cleaved with PstI, which cleaves…

A: A plasmid is an extrachromosomal small DNA molecule present in a cell and is also physically…

Q: For pGLO plasmid that contains araC gene and Ampr gene, what are the names of their promoters and…

A: pGLO plasmid  pGLO plasmid is a 5371bp genetically engineered plasmid to create genetically modified…

Q: Draw a representative agarose gel showing the bands, and the direction of current flow as well point…

A: EcoR1 is a restriction enzyme that can cleave a dsDNA at the sequence GTTAAC. They cleave the cite…

Q: When cloning a foreign DNA fragment into a plasmid, it is often useful to insert the fragment at a…

A: Recombinant DNA technology is the modern technology that is used to engineer DNA molecules that have…

Q: You want to express the protein of a newly discovered human gene using E. coli, so you clone the…

A: Protein tags are most frequently used to purify proteins for which no protein-specific antibody…

Q: A eukaryotic gene is added to a mixture containing a restriction enzyme EcoRI and a bacterial…

A: Which media allow growth of bacteria that did not take up any plasmid?

Q: How many more genes in the plasmid (besides the insulin cDNA insert) are need to determine which…

A: Insulin is a hormone that helps in reducing the level of blood sugar. In diabetic patients, the…

Q: complimentary sticky ends

A: DNA's are cut by using restriction enzymes ( endonuclease or exonuclease) which produce sticky or…

Q: A newly discovered insect has a genomic DNA that does not contain any repetitive DNA or any intronic…

A:

Q: You would like to clone your favorite human gene into an eukaryotic expression plasmid, such as…

A: Ans: The plasmid which is used for the expression of the gene in the form of protein and they have…

Q: plasmid has been modified such that it carries the gene for green fluorescent protein (GFP) under…

A: GFP protein is found in the photoorgans of Aequorea Victoria a jelly fish. It is a 28 kDa protein.…

Q: Where is the F plasmid located in an Hfr cell? Where is the F plasmid located in an F+ cell?

A: Prokaryotic cells are unicellular organisms that differ from multicellular organisms in composition…

Q: A plasmid that is both ampicillin and tetracyclineresistant is cleaved with PstI, which cleaves…

A: The tetracycline resistance gene is present in the recombinant plasmid. To select the recombinant…

Q: A plasmid has two restriction cut sites for EcoRI and one restriction cut site for HindIII. The…

A: Plasmids are the small double stranded circular DNA molecules which contain extra genetic…

Q: The gene atg-12 codes for a protein associated with abnormal rates of cell destruction and recycling…

A: Introduction : 1) Recombinant DNA Technology is a term used to describe the process of creating…

Q: You transform bacteria with a plasmid carrying the ampicillin-resistance gene ampR. How would you…

A: Antibiotic-resistant genes are used as selectable markers for the process of recombinant DNA…

Q: plasmid has a total size of 2686 bp. There is an EcoRI recognition site at position 439, BamHI…

A: Plasmid  It is an extra chromosomal piece of DNA in the cell.

Q: The gene atg-12 codes for a protein associated with abnormal rates of cell destruction and recycling…

A: It is given that atg 12 gene us responsible for producing a protein which is associated with…

Q: ________________ "cut" DNA at specific sequences and the resulting segment with sticky ends is…

A: Recombinant DNA Technology is a kind of technology that utilizes certain enzymes to cut the DNA into…

Q: In Figure 5-19, how many different bacterial species areshown as having contributed DNA to the…

A: A plasmid is an extrachromosomal circular DNA molecule found in the bacteria which is able to…

Q: Primers designing for epitope tagging: Design forward and reverse primers to amplify the following…

A: Primers should generally have the following properties: 1. Length of 18-24 bases. 2. 40-60% G/C…

Q: In an attempt to clone a gene into the TOL plasmid, it was observed that the restriction product of…

A: Restriction Enzymes or “Restriction endonucleases” are proteins that cut DNA at a specific base…

Q: You have two E. coli strains, XL10 and K12. XL10 is ampicillin resistant, or AmpR, and can grow on…

A: The plasmid is the extrachromosomal material found in bacterial cells. They contain various…

Q: When E. coli cells are mixed with recombinant vector DNA and subject to a stress such as heat shock,…

A: Prokaryotes are the single celled organisms (unicellular) and are the simplest form, which do not…

Q: A newly discovered insect has a genomic DNA that does not contain any repetitive DNA or any intronic…

A: Biotechnology is the use of our understanding of biological processes to develop useful applications…

Q: When constructing a Recombinant DNA Plasmid intended to be transformed into E. Coli, why is a…

A: Recombinant DNA plasmids are molecular tools which are utilized to deliver foreign gene into a…

Q: You have two cell cultures, each containing a different plasmid. The first plasmid is -5kbp long and…

A: Cell culture is a process in which cells are grown outside their natural environment under…

Q: Assume that a plasmid is 4700 base pairs in length and has restriction sites for a given restriction…

A: A circular piece of DNA has four sites for a particular restriction enzyme, then that restriction…

Q: In the amplicon insert you have an enzyme restriction site for NdeI at 500 bp. If you digest the…

A: Answer: RESTRICTION ENZYME = These are the enzymes or molecular tools which are used to cut the…

Q: You have two samples you have to sequence: one is a cloned plasmid that you want to verify the…

A: Sequencing of DNA refers to the biochemical methods for determining the order of the nucleotide…

Q: In the formation of recombinant DNA, a restriction endonuclease cuts a bacterial plasmid to give…

A: Recombinant DNA is a type of genetically engineered DNA which is produced to combine the best…

Q: RNA that codes for the spike protein of SARS-CoV-2 and the plasmid pET32(a+), how will you…

A: A pET-32 α (+) vector series is intended for the expression of amide sequences united with the 109…

Q: Ampicillin and tetracycline resistant genes are sometimes located on plasmids used in cloning. What…

A: Marker genes are used in Recombinant DNA technology experiments in order to differentiate between…

Q: Besides plasmid, what other vectors could you use to amplify your gene of interest? Name THREE (3)…

A: A vector is any vehicle, often a virus or a plasmid that is used to ferry a desired DNA sequence…

Q: You first need to create a plasmid. What are the minimal components necessary to develop this…

A: Since you have asked many questions, I'm giving you the answer of first 3 Please repost in order to…

Q: If you use the pUC18 vector  to clone in the MCS region, predict the following: a) Do bacteria that…

A: pUC18 [plasmid of the University of California],  is a bacterial plasmid that was derived from pBR…

Q: The total size of the plasmid is 2686 bp. There is a PstI recognition site at position 439, HindIII…

A: Answer - d. 3 fragments: 447bp, 507bp and 1732bp

Q: One of your colleagues in a molecular laboratory did not put proper labels to some of the vessels he…

A: To extract the plasmid you need to follow the below mentioned steps. 1) add isotonic solutions to…

Q: If an enhancer is placed on one plasmid and its corresponding promoter is placed on a second plasmid…

A: Plasmids are extrachromosomal DNA present in prokaryotic cells. Plasmids generally confer resistance…

Q: Which of the following options best explains how the lacZ reporter gene works when screening for…

A: Given: Need to find the  correct option among the given four options to know how lacZ reporter gene…

Q: A. What would be the donor organism to be used in this cloning? B. How would you make sure you only…

A: E.coli WA802 strain could be used as a donor organism in cloning pKY plasmid as it is compatible…

Q: A plasmid that is both ampicillin and tetracyclineresistant is cleaved with PstI, which cleaves…

A: A plasmid is a small extrachromosomal DNA molecule that exists in a cell and is physically distinct…

Q: You have set up a recombinant DNA experiment using the plasmid PBR322 as the vector (see plasmid…

A: PBR322 is a cloning vector which is widely used for cloning purposes. The map of PBR322 is given in…