A scientist seeks to synthesize a rare human protein in lab. To accomplish this, she utilizes  recombinant techniques to insert the DNA of a eukaryotic gene for the protein into bacterial  plasmids. These plasmids are transformed into bacteria for expression. She is disappointed to  discover that the gene product from the bacteria is not the correct protein. What corrective step  could she take in the procedure to fix this issue?

Q: In cancerous cells, telomeres are substantially longer then telomeres found in non-cancerous cells.…

A: Telomeres are the repetitive DNA–protein complexes at the ends of chromosomes, that are important…

Q: Describe in detail how blue/white screening works in bacteria. As part of your description explain…

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Q: Why must the gene be inserted into a vector for it to be cloned?

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Q: Although many cloning applications involve introducing recombinant DNA into bacterial host cells,…

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Q: The plasmids from the pUC series are created in the University of California. They carry a lacZ gene…

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Q: nion and burger lover, your friend would like to incorporate the cheems gene (“cheM”) from the rare…

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Q: A human gene was insterted into a plasmid and then produced by a bacterium. Unfortunately, the human…

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Q: What does the F plasmid code for?

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Q: Plasmids are the only vectors currently available for use in recombinant procedures.True or false?

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Q: If I clone a complete eukaryotic gene, including the eukaryotic promoter region, ligate it into a…

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Q: Why are nucleic acid probes necessary to lyse cells containing plasmids in order to detect the…

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Q: Explain the purpose of the antibiotic resistance gene in this experiment. Why is this genetic trait…

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Q: What is a restriction enzyme? How can restriction enzymes be used to splice a piece of human DNA…

A: BASIC INFORMATION ENZYMES They are the catalyst. They help in accelerating the chemical reaction.…

Q: If a researcher wants to use bacteria to make a eukaryotic protein, she should use a plasmid from:…

A: The recombinant DNA technology involves transfer of foreign DNA into an organism. We used this…

Q: You want to express the protein of a newly discovered human gene using E. coli, so you clone the…

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Q: When using a conventional plasmid cloning vector containing a b-galactosidase gene, it is possible…

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Q: What are the essential elements of a plasmid cloning vector? Discuss the importance of each element…

A: Essential elements of plasmid cloning vector  Presence of Origin of replication for autonomous…

Q: A plasmid vector has two genes in it, a gene for penicillin resistance (penR) and another for…

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Q: You are cloning a gene called ice, which will help strawberry plants survive in cold weather, into a…

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Q: "Plasmids are still the workhorses for many applicationsof recombinant DNA technology". Define the…

A: Introduction  Microorganisms such as bacteria, viruses, etc. are not just pathogenic and harmful but…

Q: the role (presence and absence) of a selectable marker (selection) in cloning a recombinant DNA…

A:

Q: Jane has isolated a mutant strain of yeast that forms red colonies instead of the normal white when…

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Q: There may be more than one correct answer, select all that apply.  To clone genes, a plasmid vector…

A: Introduction  A plasmid vector is a small, circular piece of DNA that is used as a tool for…

Q: If you are a genetic engineer and you cloned your gene of interest in a plasmid and you want to know…

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Q: You are on a research project to study rice. Your want to use reverse transcription PCR to amplify…

A: PCR stands for Polymerase Chain Reaction. It is a laboratory technique used to amplify or create…