Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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.Describe the method you would use to test for the uptake of a plasmid with a DNA insert
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- A.) Transformation is best described as: Group of answer choices The integration of foreign DNA into a genome The uptake of naked DNA from the environment The transfer of DNA via a bacteriophage Transfer of a plasmid from one organism to another B.) What is the function of the araC gene in the pGLO plasmid? Group of answer choices It encodes the protein that glows under ultraviolet light It allows us to select for the cells that contain the plasmid It prevents the transcription of the green fluorescent protein unless arabinose is added It ensures that the plasmid will be copied and passed on to daughter cells C.) What is the function of the bla gene in the pGLO plasmid? Group of answer choices It encodes the protein that glows under ultraviolet light It allows us to select for the cells that contain the plasmid It prevents the transcription of the green fluorescent protein unless arabinose is added It ensures that the plasmid will be copied and passed on…arrow_forwarddraw structure of plasmidarrow_forward. A second feature that virtually all plasmid vectors havein common is the selectable marker. Explain what this isand why it is such an important feature.arrow_forward
- Why do we need a plasmid to transform a bacteria? What are the essential components of a plasmid and explain their functions. What is the difference between a Plasmid and a Vector?arrow_forwardYou first need to create a plasmid. What are the minimal components necessary to develop this plasmid? In addition to these components, please draw the plasmid. In this illustration, I am looking for the components, the direction of transcription (i.e., the direction the genes will be transcribed), and what should be transcribed last? Also, where would you specifically insert the P. falciparum gene in this plasmid, and why are you checking reading frames in your gene? Finally, please name your plasmid using the correct nomenclature too. You are excited you have this new plasmid; you want to transfect it into P. marinus and provide it as an oral vaccine to laboratory mice. However, even though your supervisor enjoys your enthusiastic attitude, they do not allow you to do this quite yet because all you have is this plasmid. Why doesn’t your supervisor allow you to use the laboratory mouse for this research regarding animal welfare guidelines? Your answer should include the 3 R’s of…arrow_forwardBriefly explain the role of arabinose in the expression of the GFP gene of the pGLO plasmid.arrow_forward
- What is a plasmid? What are the minimum 3 components that a recombinant plasmid used for genetic transformation should have?arrow_forwardThe presence of colonies on the bacterial plate post transformation suggests which of the following? Group of answer choices The colonies contain the transformed plasmid and are not resistant to the antibiotic The colonies contain the transformed plasmid and are resistant to the antibiotic The colonies do not contain the transformed plasmid and are not resistant to the antibiotic The colonies do not contain the transformed plasmid and are resistant to the antibioticarrow_forward
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