Concept explainers
If the enzyme maltase has a Vo of 0.25 mM per minute when [S] = 0.10 mM, and a Vo of 0.40 mM per
minute when [S] = 0.40 mM, what is its y-intercept on a Lineweaver-Burk plot?
A. 0.10 minutes per mM
B. 0.20 minutes per mM
C. 0.50 minutes per mM
D. 1.0 minutes per mM
E. 2.0 minutes per mM
Enzymes kinetics - is the study of the reaction rate of enzyme catalyzed reactions. The reaction rate is measured with effects of varying substrate concentrations and also varying the other conditions like temp, pH, effect of inhibitors and activators.
Michaelis Menten kinetics curve is the representation of Substate concentration V/S reaction rate which is based on Michaelis Menten equation. Non-linearity of Michaelis Menten kinetics curve makes the estimation of Vmax and Km difficult, thus, researched modified the Michaelis Menten equation and developed linear graph in Lineweaver Burk plot for accurate calculation of Km and Vmax. Lineweaver Burk plot is the representation of 1/S Vs 1/Vo, i.e. reciprocal of S and Vo , that is why it is also called as double reciprocal plot. Both plots/curves are used to derived Vmax and Km values.
Vmax is the maximum velocity of chemicals reaction and Km is the substrate concentration at which half of Vmax is achieved. Higher the Km, lower the affinity of enzyme for substate and eventually high substrate concentration required for the reaction to occur.
Trending nowThis is a popular solution!
Step by stepSolved in 2 steps with 1 images
What is the x-intercept for the above reaction on a Lineweaver-Burk plot?
A. -0.5 per mM
B. -1.0 per mM
C. -2.0 per mM
D. -5.0 per mM
E. -10.0 per mM
What is the x-intercept for the above reaction on a Lineweaver-Burk plot?
A. -0.5 per mM
B. -1.0 per mM
C. -2.0 per mM
D. -5.0 per mM
E. -10.0 per mM
- You generate mutants in the metabolic pathway for starlase. You conduct some complementation tests (after testing for dominance of course) and come up with the following results: 1 2 4 6 1 + + + 2 + 3 + 4 + 5 6 a. How many complementation groups are there? [Select] b. You conduct some additional experiments to elucidate the starlase metabolic pathway. Your results are shown below. Use this information alongside information from the complementation table above to place the intermediates in the correct order on the pathway. (HINT: use the complementation groups from the table above to help you consolidate information on the tables below. Reference practice question 3 from today's lecture for help). Addition to minimal medium Mutant None starlase P 1 + + 2 + + 4 + + 5 + 6 Precursor --> [ Select ] [ Select ] [ Select ] --> starlase c. Mutant 4 has a loss-of-function mutation for which enzyme in the starlase synthesis pathway? [ Select ] E1 E2 ЕЗ Е4 Precursor > Intermediate 1→ Intermediate…arrow_forwardWhat was studied or investigated in the paper? Who would be affected by this? https://opus.lib.uts.edu.au/bitstream/10453/31887/1/2012007630OK.pdfarrow_forwardPlease help with this question with a better explanationarrow_forward
- Which of the following is true about a mixed type inhibition? a. None of these is true b. A Lineweaver-Burk plot will give parallel lines c. The lines of a Lineweaver-Burk graph will cross in the top left quadrant d. The KM will change but not the Vmaxarrow_forwardIn a-c below, will the residue on the right-hand side increase or decrease the pKa of the residue on the left-hand side? Explain.arrow_forwardIn a pUC19 digest for 1 ug of pUC19 (DNA conc. 282ng/ul) using 10X Cutsmart buffer, pure water and BamHI enzyme in a total volume of 40 ul, how much of each solution is added to the total volume?arrow_forward
- If the enzyme lactase has a Vo of 0.111111111111 mM per minute when [S] = 1.0 mM, and a Vo of 0.20 mM per minute when [S] = 5.0 mM, what is its Km? 0.125 mM 0.25 mM 0.50 mM 1.25 mM 5.0 mMarrow_forwardIf the enzyme lactase has a Vo of 0.40 mM per minute when [S] = 1.25 mM, and a Vo of 1.0 mM per minute when [S] = 5.0 mM, what is its Vmax, Km and the slope on a linewver-burk plot?arrow_forwardV2arrow_forward
- BiochemistryBiochemistryISBN:9781319114671Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.Publisher:W. H. FreemanLehninger Principles of BiochemistryBiochemistryISBN:9781464126116Author:David L. Nelson, Michael M. CoxPublisher:W. H. FreemanFundamentals of Biochemistry: Life at the Molecul...BiochemistryISBN:9781118918401Author:Donald Voet, Judith G. Voet, Charlotte W. PrattPublisher:WILEY
- BiochemistryBiochemistryISBN:9781305961135Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougalPublisher:Cengage LearningBiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage LearningFundamentals of General, Organic, and Biological ...BiochemistryISBN:9780134015187Author:John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. PetersonPublisher:PEARSON