f the enzyme lactase has a Vo of 0.111111111111 mM per minute when [S] = 1.0 mM, and a Vo of 0.20 mM per minute when [S] = 5.0 mM, what is its Km? 0.125 mM 0.25 mM 0.50 mM 1.25 mM 5.0 mM

Vo 60 50 40 30 20 mm/sec 0100 0 O 0 0 50 100 150 200 250 300 Substrate (mM) Use the Michaelis-Menten plot to answer this question. What is the estimated value of Vmax of the enzyme catalyzed reaction (square data points)? (choose the one best answer) 10

Calculate, or make a best estimate of, the unknown factors in the situations listed in  Table 1.  Table 1. Data for problem 1.    To find  Data (i)  Ek  [K+]out = 4 mM; [K+]in = 130 mM (ii)  [Cl-]in  [Cl-]out = 570 mM; ECl = -65 mV (iii)  ECl  [Cl-]out = 150 mM; [Cl-]in = 8 mM; in a mammal (iv)  [Na+]in  Overshoot of action potential = +35 mV; saline [Na+] = 112 mM (v)  [K+]in  Blood [K+] = 3.2 mM; undershoot of action potential = -87 mV (vi)  ECa  [Ca2+]out = 5.6 mM; free [Ca2+]in = 0.8 mM (vii)  [K+]out  [K+]in = 350 mM; Ek = -82 mV

If the enzyme lactase has a Vo of 0.40 mM per minute when [S] = 1.25 mM, and a Vo of 1.0 mM per minute when [S] = 5.0 mM, what is its Vmax? Calculate the Km of the above enzyme. Calculate the slope on a Lineweaver-Burk plot (Km / Vmax) for the above enzyme.