Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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HOw do I plot on michealis Menten plot and a lineweave burk plot and how do I solve the Km and vmax for both graphs. can you give me an example please on how i should solve it?
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Step 1
Michaelis-Menten plot is a representation of substrate concentration [S] with the velocity of an enzyme catalyzed reaction (Vo). This curve is a hyperbola in which as the substrate concentration increases, the velocity of the reaction increases in an exponential manner followed by a linear relation and then, a plateau region at which velocity remains unchanged with increase in substrate concentration.
Vmax is the maximum velocity of the reaction.
Km is defined as the substrate concentration at which velocity of the reaction is half maximal (Vmax/2).
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