n a pUC19 digest for 1 ug of pUC19 (DNA conc. 282ng/ul) using 10X Cutsmart buffer, pure water and BamHI enzyme in a total volume of 40 ul, how much of each solution is added to the total volume?

Q: Suppose you calculate that you can load 2000ng of Sample A and suppose your concentration of Sample…

A: * We must figure out how much Sample B is needed to give 2000ng of protein in order to compute the…

Q: How to create 50 uL of TER (with compostions 10 mM Tris-HCl, pH 7.4; 1 mM EDTA, pH 8; 100 ug/mL…

A: The following steps will describe the calculations for the creation of 50 µl of TER.

Q: You have an aqueous solution of hemoglobin (pl = 6.8) at pH 6.5, and currently the proteins are…

A: pH affects the ionisation of amino acids and can thus influence the nature and distribution of…

Q: A 100 ml of a 1% sol'n of boric acid (freezing point = -0.29) isotonic w/ blood by NaCl (freezing…

A: Asked : Grams needed to make isotonic solution for given conditions

Q: A certain plant storage protein, after being isolated, was digested using both 8N H2SO4 and conc.…

A: Paper chromatography is an analytical technique that separates specific substances from a mixture.…

Q: Enzyme Combination - Cla I + EcoRI  What is the number of fragments? What are/is the size(s) of…

A: The restriction endonuclease are specific enzyme that are responsible for cutting double strength…

Q: Given a stock protein solution with a concentration of 3 mg/ml, determine the protein concentration…

A: Stock solutions are used in many laboratory procedures, in order to save space for keeping larger…

Q: "The native structure of hemoglobin (HB) comprises of two α and two β subunits, each of which…

A: Given: The native structure of hemoglobin (HB) comprises two α and two β subunits, each of which…

Q: A large chaperone protein complex GroEL is approximately 16 nm in diameter. When it is dissolved in…

A: Particles present in cells show rapid movement(back and forth) and we call it Brownian motion. The…

Q: Proteins pl Fibrinogen 5.8 Hemoglobin 7.1 Lysozyme 11.0 Pepsin 1.0

A: Elution: - It is the process of separating one substance with the other by the process of washing…

Q: Given the following information, which protein would be most difficult to isolate from protein X…

A: Salting and dialysis are the processes used to isolate proteins. Salting is a purification process…

Q: From this standard curve and chart below, does the separation of molecules in the mixture appear…

A: Size exclusion chromatography is a technique used to separate molecules by the virtue of their…

Q: Amino acid analysis of the peptide angiotensin II shows the presence of eight different amino acids…

A: Trypsin and chymotrypsin are proteolytic enzymes, which help to cleave the peptide bonds from…

Q: 1. Lactoferrin is an iron-binding glycoprotein that forms the antibiotic fragment lactoferricin…

A: Ammonium sulphate is the commonly used salt for protein precipitation and extraction. Under high…

Q: If the absorbance at540nm of a biuret and protein solution containing 0.25mg/ml of protein .24 the…

A: The biuret assay is a colorimetric method used to quantify the concentration of proteins in a…

Q: Why does a dual layer of Cr/Au (20/100nm) delaminate during a GSTAT electrochemical polymerization…

A: Galvanostatic Polymerization with a Static Template (GSTAT) is a polymerization technique used for…

Q: B. For each of the following amino acid sequences in the protein, predict the most likely secondary…

A: "Since you have asked multiple questions, we will solve the B question for you. If you want a…

Q: Bovine ribonuclease folds with ΔH° = -280 kJ mol-1 and ΔS° = -0.79 kJ mol-1 K -1. Assume ΔH° and ΔS°…

A: The basis for predicting spontaneity of a reaction is based on (ΔG), the change in G, at constant T…

Q: suctose density gradient ultractifugation is a powerful technique for fractionating macromolecules…

A: The sucrose density gradient is used for fractionating the DNA, RNA, and protein. The samples are…

Q: Give two benefits of utilizing the biuret reaction to assess protein concentration over directly…

A: A Biuret test is a generic test performed by molecules having two or more peptide links (CO-NH…

Q: hypothetical

A: Isolation and characterization of plant and human pathogenic bacteria from green pepper (Capsicum…

Q: The

A:

Q: Electrophoresis is performed at PH 6.8 on a mixture of mutated hemoglobin that differ from normal…

A: To determine the order of migration between the cathode and anode of the mutated hemoglobins…

Q: what is the dna concentration suspended in PO4 buffer at ph 7.0 that has an absorbance at 260 nm…

A: DNA concentration of a sample can be determined using spectrophotometry and measuring the absorbance…

Q: Figure 3: ● • . ● KDa ● 97.4 66.2 45.0 ● 31.0 21.5 14.4 S-1 p-1 2.8 2-d S.3 p-3 This figure was…

A: The study of protein behavior under different conditions is a fascinating aspect of molecular…

Q: An oligopeptide ALVGALGATPTPQMWSHSWRGVSIKS was digested with trypsin.Which method would be most…

A: CHROMATOGRAPHY It is a technique of separation of molecules on the basis of solubility into two…

Q: Describe the concept of isoelectric point and calculate the PI for isoleucine?

A: Isoelectric point is the pH value at which the charge on an amino acid is zero.

Q: You repeat your kinetic assay for three additional substrate concentrations and determine the…

A: Michaelis-Menten Kinetics is one of the simplest approaches to enzyme kinetics.The basic equation is…

Q: A gel filtration column with a fractionation range of 1.5-20 kDa is used to separate out the…

A: Gel filtration chromatography is a technique that is used to separate molecules based on their…

Q: 1. The graph given represents a size-exclusion chromatogram after the refolding of the hemoglobin…

A: Chromatography is a technique of protein purification in which protein can be separated based on its…

Q: An antibody binds to another protein with anequilibrium constant, K, of 5 × 109 M–1. When it binds…

A: The equilibrium constant, K, expresses the relationship between a reaction's products and reactants…

Q: If a pediatric vitamin contains 1500 units of vitamin A per milliliter of solution, how many units…

A: A vital nutrient that is fat-soluble and plays a role in many bodily processes is vitamin A.It is…

Q: Serum Albumin (Monomer) Ovalbumin 66,000 43,000 32mm 39mm 60mm 26mm 14,400 Lysozyme Unknown 1 ?…

A: Distance Molecular weight 23 132000   32 66000   39 43000   60 14400…

Q: please calculate the unknown concentration of the protein A wih an absorbance value of A188 given…

A: Absorbance = -log (I / Io) where I is intensity of transmitted light and Io intensity if incident…

Q: KDa 97.4 66.24 45.0 31.0 21.5 14.4 8-1 1-d s-2 For condition 1 - For condition 2 - For condition 3 -…

A: This question is asking about the dissemination of proteins within the supernatant and pellet…

Q: In a TPN bag contains: Kcal of 1000 ml of D50 W, 1000 ml of 7% amino acids, and 650 ml of 20%…

A: Exact Calorific Infusion into the dietary pattern of critically ill patients is essential for a…

Q: @) so this eniyme catalytically pufet ? Explain your keasoning. , ik (b) What is Vmaz, |-4 nomol /me…

A: a) Given, K1 = 1.4 X 108 M-1s-1                K2 = 2.5 X 105 s-1               K3 =  1.5 X 103 s-1…

Q: [10'] For a bacteriophage T7, the following data at zero concentration have been obtained:…

A: In order to find the molecular mass, first we need to convert the Sedimentation coefficient value…

Q: In a molecular disease of hemoglobin, Hemoglobin Rainier, Tyr 145β is replaced by Cys, which forms a…

A: Hemoglobin is a protein present in the red blood cell. The hemoglobin function is to transport the…

Q: r^2=6Dt , where D is the diffusion coefficient of thediffusing object  and t is the  time that the…

A: The objective of the question is to find out the time it takes for a protein to diffuse across a…

Q: 1.0.1 mL of a protein solution of concentration of 11 mg/mL was diluted to a total volume of 4.0 mL…

A: The four types of biological macromolecules are nucleic acids proteins lipids and carbohydrates.…

Q: You run 5 standard proteins listed below on a size-exclusion (gel filtration) column with limit of…

A: Size-exclusion chromatography is a separation technique where the proteins are separated based on…

Q: Bacteria have equivalent diameters of 2x 10-6 m and densities of approximately 1 kg/L. Under optimal…

A: To determine the mass of bacteria that would accumulate in 72 hours under optimal growth conditions,…

Q: Please describe the protein purification process with the aim of purifying a protein which locates…

A: We will answer the first question since the exact one was not specified. Please submit a new…