Using the graph data I estimated Slope = 2.8, about the same as the steeper part of the maternal hemoglobin, does this represent a strong positive cooperativity between enzyme subunits? pO₂10152025303550100Y0.20.40.60.80.850.90.951.0log pO₂1.001.181.301.401.481.541.702.00log (Y/(1-Y))-0.60-0.180.180.600.750.951.28undefined log (Y/(1-Y))1.51-0.5-10.5Fetal Hemoglobin Hill Plot1log po₂1.522.55. Estimate the slope(s) of the Hill plot, for the portion(s) that is (are) clearly linear. This numberis the Hill coefficient, symbolized n or ní. For instance, for the maternal hemoglobin plot above,the Hill coefficients are 1.0 and 2.8 (the steeper part in the middle). If the Hill coefficient = 1.0,then there is no cooperativity between enzyme subunits. If the coefficient is greater than 1.0,there is positive cooperativity. If the coefficient is less than 1.0, there is negative cooperativity.Comment on the type and degree of cooperativity shown in the fetal hemoglobin, compared tothe maternal hemoglobin.

Hemoglobin (Hb) is composed of 4 polypeptide chains. Adult Hb has 2 alpha chains and 2 beta chains…

Answered: Using the graph data I estimated Slope…

Using the graph data I estimated Slope = 2.8, about the same as the steeper part of the maternal hemoglobin, does this represent a strong positive cooperativity between enzyme subunits?

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

Using the graph data I estimated Slope = 2.8, about the same as the steeper part of the maternal hemoglobin, does this represent a strong positive cooperativity between enzyme subunits?

pO₂
10
15
20
25
30
35
50
100
Y
0.2
0.4
0.6
0.8
0.85
0.9
0.95
1.0
log pO₂
1.00
1.18
1.30
1.40
1.48
1.54
1.70
2.00
log (Y/(1-Y))
-0.60
-0.18
0.18
0.60
0.75
0.95
1.28
undefined

log (Y/(1-Y))
1.5
1
-0.5
-1
0.5
Fetal Hemoglobin Hill Plot
1
log po₂
1.5
2
2.5
5. Estimate the slope(s) of the Hill plot, for the portion(s) that is (are) clearly linear. This number
is the Hill coefficient, symbolized n or ní. For instance, for the maternal hemoglobin plot above,
the Hill coefficients are 1.0 and 2.8 (the steeper part in the middle). If the Hill coefficient = 1.0,
then there is no cooperativity between enzyme subunits. If the coefficient is greater than 1.0,
there is positive cooperativity. If the coefficient is less than 1.0, there is negative cooperativity.
Comment on the type and degree of cooperativity shown in the fetal hemoglobin, compared to
the maternal hemoglobin.

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