Hello! Can you please help me with the following: a.    Draw the basic batch growth curve and label the four difference phases of growth and kinetics on the curve. b.    Give a description of each phase on the curve c.    Compare (less than, greater than, equal to, equal to zero) growth rate (Rg) and death rate (Rd) at each phase.

. A certain medium has the following composition: Glucose                                   15 g Yeast extract                           5 g Peptone                                   5 g KH2PO4                                 2 g Distilled water                        1,000 ml Tell what chemical category this medium belongs to, and explain why this is true. How could you convert Staphylococcus medium (table 3.6A) into a nonsynthetic medium?   a. Name four categories that blood agar fits. Alpha hemolysis Beta hemolysis Gamma Alpha prime Name four differential reactions that TSIA shows. Observe figure 3.15. Suggest what causes the difference in growth pattern between nonmotile and motile bacteria. Explain what a medium that is both selective and differential does, using figure 3.18. a. What kind of medium might you make to selectively grow a bacterium that lives in the ocean? MSA Mannitol salt agar One that lives in the human stomach? Why are intestinal bacteria able to grow…

Can I get help on finding the appropriate amount?

Please help me with these two questions. Thank you so much.

Batch culture time prediction: A strain of Escherichia coli has been genetically modified to produce human protein. A batch culture is started by inoculating 12 g of cells into a 100 liters bubble column fermenter containing 10 g/l glucose. The culture does not exhibit a phase lag. The maximum specific growth rate of the cells is 0.9 h^-1, the glucose biomass yield is 0.575 g/g. Estimate the time required to reach the stationary phase.

Match the types of data to the example.                                                                                                         Precise measurements                                                                                                         Assay standard                                                                                                         Positive control                                                                                                         Quantitative results                                                                                                         Negative control                                                                                                         Qualitative results                                                                                                         Standard curve…

Develop a spreadsheet to predict as functions of time with concentration, volimme and permeate flows for a batch ultrafiltration. Assume that the flux depends on concentration as follows: J(L/M²/H) = A*EXP(-bC) where C is the retentate concentration in g/l. Assume that the recirculation rate is kept high enough that shear rate dependence is eliminated. Do a sample run with C.= 0.5 g/l, Cfinal = 50 g/l, A = 50, and b = 0.02. Assume a filtration area of 19.4M² and an initial volume of 3255 L. Assume σ (protein) = 1.0 or σ (protein) = 0.95 or σ (protein) = 0.90.

Please answer fast Dilution Problem. A culture of Staphylococcus is diluted as follows: (1) 20mL are added to 80mL of water. (2) 10uL from (1) are then added to 9.99mL of water. (3) A 10-2 dilution is made from tube # (2). (4) 100uL from (3) are plated for a pour plate and incubated.   Growth Problem.A culture with approximately 2x103 cells/mL were incubated. After 3 hours, the number of cells had increased to 3x105. a) How long was the generation time in minutes?b) How many generations have occurred

Determine the minimum inhibitory concentrations (MIC) for compounds 1, 2, and 3 based on the microdilution results.

Part a. You making 30 mo of agarose gel. Sybersafe A concentration 1000x. How much you will add......ul to the gel. b. You will be loading 20 your diagnostic digest onto your gal. The loading dye you have been provided is 5x concentrated. You will add..... ? ul to your sample before loading your gel.