a. You making 30 mo of agarose gel. Sybersafe A concentration 1000x. How much you will add......ul to the gel.

I need help with my homework this question is very confusing How long would it take for a solution made by diluting 2.5 ml of #1 with 7.5 ml of H2O to react with 10 ml of #2?

GELATIN b.how will you inoculate? c.How will you incubate? d. what are Post- Incubation procedures? e. How will you read the positive and negative results?

5. The proteins listed below are separated by (1) isoelectric focusing (IEF) followed by (2) sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE). A single sample of the protein mixture is placed at the top center of the gel to begin step 1. ID 1 2 3 4 5 6 7 8 Protein Collagen y-Globulin Insulin Fibronectin Lysozyme Pepsin Serum Albumin Myoglobin Molecular Weight 300,000 150,000 5,700 220,000 14,300 34,500 66,500 16,700 Conc pl 0.5 mg/L 4.7 1 mg/L 7.2 12 mg/L 5.3 5.8 11.0 5 mg/L 2 mg/L 10 mg/L - added prior to running IEF? left out of the process entirely? 4 mg/L 2 mg/L 1.0 4.9 7.1 A. Which of these proteins would electrophoresis NOT separate effectively? B. Which of these proteins would isoelectric focusing NOT separate effectively? Type fibrous a-helices globular protein globular protein fibrous protein enzyme digestive enzyme blood protein muscle protein C. Show the expected results after each step, by drawing qualitative diagrams similar to those shown in the class…

Phenol Red Test Question: Assuming you tested an organism using Phenol Red glucose and Phenol Red Base broths, which of the combinations of results in the following table would be reliable. Interpret each of the combinations and explain how the results might be obtained

Oops!  Your pipettor wasn’t set correctly and you only pipetted 87 µl of a 10-7 dilution when plating serial dilutions.  But, you noted this error in your lab notebook so you could correctly perform the calculation.  When this plate was grown, you counted 147 colonies on the plate.  What was the concentration of bacteria in the original, undiluted suspension?

how to make 100ml of TBST buffer from 1X TBS (tris buffered saline solution) and 0.1% tween-20. I am just confused how to do the calculations when it is 1X TBS and not 10X TBS. Please show calculations